BACKGROUND AND PURPOSE:It has been reported that high-resolution susceptibility-weighted imaging (HR-SWI) is a promising tool for assessing brain tumor characterization noninvasively. The purpose of this study was to determine the added value and diagnostic performance of HR-SWI for differentiating solitary enhancing brain lesions (SELs) by assessing intratumoral susceptibility signals (ITSSs).
Dental composite resin restoration for defective tooth may lead unpolymerized resin monomers to be leached into dental pulp tissue. The aim of this study was to investigate the early gene expression change over time of human dental pulp cells (HDPCs) treated with a low-level toxic concentration of Triethylene Glycol Dimethacrylate (TEGDMA), a common dental resin monomer, by adopting the novel high-throughput transcriptome analysis of RNA-seq. The low-level toxic concentration of TEG-DMA was determined through MTT assays with serially diluted concentrations. After the HDPCs were exposed to TEGDMA for 6, 12, 24 or 48 hr, the total RNA of the samples was prepared for RNA-seq. qRT-PCR for several genes was performed for validation of RNA-seq results. In the treated group, 1280 genes were differentially expressed compared with the control group. Five patterns of time-series gene expression profiles were identified through k-means clustering analysis. Angiogenesis, cell adhesion and migration, extracellular matrix organization, response to extracellular stimulus, inflammatory response and mineralization-related process were major gene ontology terms in functional annotation clustering. HMOX1, OSGIN1, SMN2, SRXN1 AKR1C1, SPP1 and TOMM40L were highly up-regulated genes, and WRAP53 and CCL2 were highly down-regulated genes over time. qRT-PCR for several genes exhibited a high level of agreement with RNA-seq. TEGDMA induced the HDPCs to show massive and dynamic gene expression changes over time. The previously suggested toxic mechanism of TEGDMA was not only verified, but new genes whose functions have yet to be determined were also found.
This study tested the hypothesis that dentin bonding agents (DBAs) cured with a light emitting diode (LED) light curing unit (LCU) would not show a statistically significant different dentin shear bond strength (DSBS) relative to a halogen LCU, when the LED and halogen LCUs had a supposedly similar irradiance. Five commercial DBAs were tested: ScotchBond Multipurpose, Single Bond, One Step, Clearfil SE Bond, and Adper Prompt. The LCUs used in this study were a VIP (Bisco) for the halogen light and an Elipar FreeLight (3M ESPE) for the LED. First, the emission spectrum and spectral irradiance of both LCUs were investigated. Next, the DSBS of the DBAs cured with each LCU was measured. Two-way analysis of variance was used to analyze whether there were differences in DSBS resulting from the type of LCU and DBA used. The halogen LCU showed a higher total irradiance value relative to the LED LCU, but the difference of spectral irradiance was reduced in the efficient wavelength ranges for camphoroquinone activation. Although the DBAs cured with both types of LCUs showed similar DSBS values, Clearfil SE Bond showed the highest DSBS value when cured with a halogen light. Therefore, the null hypothesis of this study was rejected, indicating that the curing effectiveness of the LED LCU on some DBAs can be lower than that of a halogen LCU in terms of bond strength.
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