Gynogenesis of Chinese long cucumber (Cucumis sativus L.) was induced in unpollinated ovules cultured on cucumber basal medium (CBM) supplemented with thidiazuron (TDZ) and in some experiments AgNO 3 . High induction frequencies (7.85 -12.14 %) were found in unpollinated ovules at the time of anthesis and at 0.03 -0.07 mg dm -3 TDZ. Histological analysis indicated that embryo sacs developed completely at the time of anthesis. Further, the highest plant regeneration rate was achieved at CBM supplemented with 0.05 mg dm -3 a-naphthaleneacetic acid, 0.2 mg dm -3 6-benzyladenine, and 5 -10 mg dm -3 AgNO 3 . Flow cytometry analysis showed that 80 % of the regenerated plants were haploid. Histological micrographs and ploidy level analyses clearly revealed initiation, development, and germination of embryos from the unpollinated ovules.
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