A method is described that extracts fluoride selectively as trimethylfluorosilane using carbon tetrachloride and trimethylchlorosilane . Drawbacks of other methods as well as advantages of the proposed method are discussed. The method is directly applicable to plasma and faeces samples, has a short gas chromatographic run time due to the use of a backflush technique and is suitable for automation. Its lowest quantifiable limit for plasma and faeces is 10 micrograms/l and 1 microgram/g fluoride respectively, using 1.0 ml or 1.0 g samples. Preliminary pharmacokinetic parameters of fluoride obtained in two subjects are reported.
A fully automated system is described in which a gas chromatograph equipped with a backflush valve is automatically operated under the control of a specially designed timer unit. The system requires minimum data acquisition due to the selection of the signals to be integrated while waste of recorder paper is also avoided. It has the capability of unattended operation of fifty samples which--in the case of fluoride determination--are chromatographed in about four hours.
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