We studied CO 2 exchange and phenolic compound production in various organs of unhardened and hardened winter wheat ( Triticum aestivum L.) plants. The rates of CO 2 assimilation at saturating illumination (photosynthesis) and CO 2 evolution in darkness (respiration) declined substantially at the autumnal decrease of ambient temperature. However, because of a higher cold resistance of photosynthesis, the ratio of photosynthesis to respiration rates increased 1.5-fold. These gas exchange changes were accompanied by the accumulation of total soluble phenolics in leaves and a polymeric phenolic compound lignin in roots. We did not observe any changes in the production of either soluble or polymeric (lignin) phenolics in crowns.
The effect of different concentrations of lead was investigated in two different ways in the leaves of Helianthus annuus plants. In the first way, removed leaf discs from 5-wceks old plants were floated in 0.05, 0.25, 2.50 and 5.00 mM PbNO3 solutions for 2, 4 and 6 days. In addition, the chlorophyll content and the activity of peroxidase enzyme which are the parameters of stress conditions in plants were also measured. In this experimental series, a decrease was determined in polyamine and chlorophyll contents and in the peroxidase activity in leaf discs which were treated with different concentrations of lead. In the second experimental series, one half of the upper leaves of 5-wceks old plants were used for lead treatments, while the other half for control. In half leaf experiments, lead in 0.05, 1.25 and 5.00 mM concentrations were sprayed to the both sides of the half leaves. According to the results of the half leaf experiments, 0.05, 1.25 and 5.00 mM lead treatments increased the putrescine content in 27, 15 and 9 % respectively. On the other hand, chlorophyll content and the activity of peroxidnse enzyme were decreased with the treatments of lead.
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