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Materials and Methods Isolation of pathogen and maintenance of biocontrol agents A. solani taken from infected tissue of diseased fruits of tomato were inoculated on water agar medium (2%; 2 g agar/100ml of sterile distillate water) and incubated at 25°C for 3 days. After incubation, single spore colony was transferred to the Petri dishes containing Potato Dextrose Agar (PDA) to obtain pure culture of the pathogen. The commercially released endophytic bacterial strains B. Subtilis (EPCO16 and EPC 5) isolated from coconut and cotton respectively and the rhizobacterial strains of P. fluorescens (Pf1, Py15 and Fp7) were obtained from the culture collection of

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