References1 Brada SJL, de Wolf JThW, Hendriks DW, Smit JW, Vellenga E.CD34 + /CD36 − cells from myelodysplasia patients have a limited ResponseOne of the main characteristics of myelodysplasia is anemia, despite the fact that a normal or increased number of normoblasts are observed in the bone marrow. To study whether this discrepancy might be in part due to a defect in the proliferation and differentiation of erythroid progenitors, an in vitro culture system was used with CD34 + /CD36 − sorted bone marrow cells of myelodysplasia patients. Selection of this subpopulation of hematopoietic stem cells provides the opportunity to enrich for the BFU-E colony forming cells. 1 Moreover, the CD34 + /CD36 − sorted bone marrow cells can selectively differentiate in the in vitro suspension culture assay along the erythroid lineage in the presence of erythropoietin and mast cell growth factor. 1 By using these assays we demonstrated a strong impairment in the proliferative response of CD34 + /CD36 − sorted bone marrow cells of myelodysplasia patients. 2,3 In contrast, the sorted CD34 + /CD36 − bone marrow cells differentiated in the in vitro suspension culture assay to a certain degree along the erythroid lineage. 3 These results demonstrate that by using highly selective cell populations, important information can be obtained with regard to the proliferative and differentiative capacity of erythroid progenitors of myelodysplasia patients. However, these data were not of predictive value for the in vivo erythroid kinetic data measured with ferrokinetic studies. 4 COMMENTS ON PUBLISHED PAPERS Inaccuracy and vested interests -two threats to etiologic research TO THE EDITORAlexander and Greaves in their report 1 focus on two main points. The first is the hypothesis of an infectious cause for childhood leukemia. While this hypothesis has been put forward in several previous publications of these authors, it was not a major issue on the Workshop. In particular, none of the
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