En el presente estudio fue evaluado in vitrio el efecto antimicrobiano del hipoclorito de sodio al 1% y del gluconato de clorhexidina al 2% como soluciones irrigadoras utilizadas durante la terapia de endodóntica. El análisis microbiológico fue realizado utilizando conos de papel absorbente esterilizados, los cuales fueron introducidos en los conductos, transferidos para caldo BHI y analizados macroscópicamente con relación al crecimiento bacteriano. Los resultados mostraron que ambas soluciones impidieron el crecimiento bacteriano. Se concluye que las soluciones de hipoclorito de sodio al 1% y de clorhexidina al 0.2% poseen acción antimicrobiana satisfactoria durante la preparación in vitrio del sistema de consuctos radiculares infectados con las cepas estudiadas.
intoxicación en perros por soda cáustica (NaOH) es la primera comunicación en Uruguay.
Aerial parts of Achillea aspleniifolia Vent. (Asteraceae), belonging to the Achillea millefoltwn group. are widely used in folk medicine for the preparation of teas with anti-inflammatory activity. Infusa (OAB.) contain the same undestroyed proazulenes as found in dichioromethane extracts. Therefore a crude proazulene fraction (purity 75%) was isolated from diploid A. asplenifolia by means of dichioromethane extraction and repeated column chromatography on silica gel.The fraction showed a remarkable dose-dependent anti-edematous effect and appeared to be more active than matricine used as reference substance.Further purification of the proazulene fraction by preparative RP-HPLC followed by filtration over silica gel cartridges allowed the isolation of the main proazulenes. The structure of the first has been confirmed by MS and 2D-NMR experiments as 4a-hydroxy-6a-angeloyloxy-9a-acetoxy-5aH, 7 a H,8j3H, 1 laFI-guai-1(10),2-dien-7,8-olide (1). HC OR1 H O 7-;fro OR2 CH3 1 R1 acetyl, R2 arigetoylProazulenes and related compounds are claimed to be responsible for the anti-inflammatory activity of teas prepared from aerial parts of species belonging to the Achillea rn/ilefolium group (1, 2, 3). Only A. millefolium ssp. collina Becker was investigated so far with emphasis on the sesquiterpenoides; achillicin (a proazulene), 8-angeloyloxyartabsin, and 2,3-dihydrodeacetoxymatricin were identified (4, 5).In order to obtain more information about that type of compounds in other species of the A. miliefolium group, a study on the diploidAchillea setacea W. & K. has been started.From dichioromethane extracts of the aerial parts, three a-methylene-y-lactones were isolated by means of repeated column chromatography, preparative TLC over silica gel, and preparative RP-HPLC followed by filtration over silica gel cartridges. The structures were determined by MS and 2D-NMR experiments as rupicolin A (1), rupicolin B (2), and dehydrodeacetylmatricarin (3). CH2 HO --OH H3C H: CH2 0 2Both 1 and 2 are found undestroyed also in infusa (OAB) prepared from A. setacea. These substances are transformed to greenish-blue compounds by steam distillation and by thermal treatment using the TAS-method. Therefore they are responsible for the green colour of the essential oil obtained from A. setacea. The compounds are the first a-methylene-ylactones found in species belonging to theA. millefolium group.According to the close structural relationship to matricin, an anti-inflammatory activity of these substances may be expected. Due to the exocyclic methylene group, the possibility of an allergenic potency of compounds 1, 2 and 3 must be considered.
La incidencia de canales ovinas con agentes extraños es significativa en el Uruguay: cada dos canales, una está contaminada. Las condiciones de los camiones y la forma en que se comercializan los corderos agrava el problema. El objetivo de este trabajo fue estudiar en un diseño completamente aleatorizado con arreglo factorial de tratamientos el efecto de la posición en el camión: PC (nivel superior o inferior) y del sistema de lavado con agua fría a presión (no lava: NL, lava llegada: LL o pre-sacrificio: LPS) sobre la contaminación (aerobios totales, Enterobacteriacea y E. coli) de 80 corderos. La interacción PC x LL resultó significativa (p = 0,05) para aerobios totales (4,48; 3,97; 4,52 y 4,61 número de colonias log 10, para canales de corderos transportados arriba lavados y sin lavar ó abajo, lavados y sin lavar, respectivamente). Mientras que la interacción PC x LPS lo fue para Enterobacteriacea (2,76; 2,25; 2,53 y 2,53, número de colonias log 10, para corderos transportados arriba lavados y sin lavar o abajo, lavados y sin lavar, respectivamente; p = 0,04) y E. coli (324, 59, 110 y 253 número de colonias/cm2 para corderos transportados arriba lavados y sin lavar o abajo, lavados y sin lavar, respectivamente; p = 0,0001). Los resultados muestran que el lavado sólo se justificaría cuando los corderos son transportados en el piso inferior, particularmente para E. coli, aunque sólo los corderos del piso superior y que no se lavaron mostraron conteos de microorganismos aceptables.
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