Tamil Nadu Agricultural University Proteome DataBase (TNAU PDB) -Black gram proteome is an open accessible database that focuses on proteome of Black gram (Vigna mungo L.). Currently, the database contains reference maps of Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of proteins obtained from artificially aged black gram seeds of variety TNAU blackgram CO 6, which is compared to that of fresh seeds. The database provides information about experimentally identified properties, such as molecular weight, pI value, of the differentially expressed protein due to accelerated ageing and protein sequences obtained using MALDI-TOF mass spectrometry. This database runs on WAMP server with HTML as the front end and MySQL as the backend using PHP as interface and it is hosted in TNAU genomics domain. The basic intention of this database is to provide the detailed information about proteome of black gram. This will help us to understand adaptive and general protective mechanism related to seed aging and effect of ageing on germination.
The results of the seed development and maturation studies in ambrette revealed that the pods and seeds attained physiological maturity on 35 days after anthesis as evidenced by the maximum dry weight of pod (2.694 g) and seed (12.63 mg) combined with minimum moisture content of pod (23.45%) and seed (21.64%). The change in colour of pod and seed from green to brown could be considered as a visible index of maturity. The seed quality parameters viz., germination (41%), 100 seed weight (1.468 g), seedling length (16.3 cm) and drymatter (8.0 mg) and vigour index (668) were also maximum at 35 days after anthesis.
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