In the present study, 20 fungal strains were isolated from tomato rhizosphere of Senegal. Of 20 strains, five showed the chitinolytic activity on chitin agar medium. Of the five strains, NG4 showed the maximum solubilization zone. This strain was identified by preliminary biochemical and 18S rRNA sequencing analysis. Enzyme production started after 3 days of incubation and maximum was observed after 5 days of incubation. Culture filtrate amended with 0.1% colloidal chitin was used in the production medium. The optimum conditions for maximum chitinase activity are -6 days of growth and temperature of 30°C at pH 6.0. The chitinase activity was also influenced by the addition of carbon and nitrogen sources in the production medium.
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