141Four Gram-negative, non-sporulating, aerobic, obligate thermophilic bacteria, isolated from non-thermal environments by enrichment with n-heptadecane as substrate, utilized n-alkanes, carbohydrates and organic acids as sole source of carbon and energy and also grew on complex media. The growth rate of these organisms, when utilizing n-heptadecane as substrate, was markedly increased by adding a low concentration (7.5 mg 1-l) of yeast extract. They grew optimally between 55 and 65 "C, and at a pH between 6.2 and 7.5. The mol yo G + C for all was between 5 1 and 58. On the basis of the amino acid and amino sugar compositions of their peptidoglycan, these organisms and other Gram-negative thermophilic bacteria can be divided into four distinct groups. Group A includes the newly isolated hydrocarbon-utilizing bacteria which have nearly equimolar amounts of glutamic acid, alanine, diaminopimelic acid and glucosamine. Group B consists of obligate hydrocarbonutilizing microbes that have lower molar ratios of glutamic acid and diaminopimelic acid, and contain either ornithine or lysine. The previously isolated non-hydrocarbon-utilizing therinophiles (K-2, Thermus aquaticus YT-1, Thermus X-1) and a newly isolated organism from a hot spring comprise group C and contain glycine, ornithine, no diaminopimelic acid, and much lower molar ratios of glutamic and muramic acids than in groups A and B. Thmnowicrobiurn roseurn lacked peptidoglycan and is placed separately in group D.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.