No abstract

Lipid profiles were estimated in two groups of normal healthy women comprising 39 non-pregnant women and 67 pregnant women at 28 and 32 weeks gestation and 6 weeks after delivery. Triglycerides, total, HDL and LDL cholesterols were high during pregnancy. Except for LDL cholesterols which remained constant, all the above decreased at 6/52 postnatally, but levels were still above those of normal non-pregnant women. Compared to the later, the atherogenic index, LDL/HDL cholesterol ratio was unchanged during pregnancy but significantly increased at 6/52 postnatally. These results may suggest that while total lipid levels increase during pregnancy, this is balanced by an even distribution of the lipoprotein fractions. After delivery, though lipid levels had decreased, the decrease in HDL cholesterol and increase in LDL cholesterol caused unfavourable changes in lipid-lipoprotein ratios. These changes may be due to the change in metabolism of the pregnant women as well as diet during and after pregnancy.

The process of micro-insemination by single or multiple sperm transfer into the perivitelline space (PVS) or by direct sperm injection into oocytes was examined by transmission electron microscopy. Spermatozoa from normal and oligozoospermic men were injected into oocytes, obtained from consenting IVF patients, mostly by zona-puncture using micromanipulators. Spermatozoa were washed by the Percoll or Ficoll methods and capacitated using Whittingham's T6 or modified Tyrode's medium or incubated in strontium medium before injection. The women were stimulated by three IVF methods and oocytes were recovered by laparoscopy or ultrasonography. Sixty-one oocytes were cultured in T6 or Ham's F-10 media (3-24 h) and were subjected to micromanipulation. Four oocytes were also studied after zona-drilling. Normal 2-pronuclear ova were developed after single-sperm transfer satisfying all morphological criteria of fertilization. Both monospermic and polyspermic fertilization resulted after multiple sperm transfer, indicating that a vitelline block to polyspermy may exist in humans. The majority of oocytes examined were unfertilized. Spermatozoa with intact or reacted acrosomes and those undergoing the acrosome reaction were found in the PVS and in the ooplasm. Abnormal spermatozoa were also seen in these locations. Quantitation of acrosomal status in 16 oocytes after multiple-sperm transfer, revealed that 24% of spermatozoa were acrosome-reacted or reacting in the PVS following Ficoll entrapment, while 76% of spermatozoa were intact (33% of these abnormal). Sperm transfer seemed to be the least invasive, while direct sperm injection was comparatively destructive to oocytes. Drilling with acid made larger breaches in the zona when compared with mechanical perforation and spermatozoa occasionally escaped through breaches. Three 2-pronuclear ova obtained after multiple sperm transfer have resulted in two pregnancies, in cases of severe oligozoospermia, during the course of this study.

In 27 women, uterine activity in the third stage of labour was correlated with blood loss measured quantitatively during the same period of time. Myometrial activity was reflected by total intrauterine pressures measured using a Gaeltec® catheter tipped pressure transducer inserted transcervically within 5 min of delivery of the placenta. Blood loss over the same 2-hour period was collected on absorbent paper and measured in the laboratory by colorimetric measurement of the haemoglobin content. As total uterine activity in the third stage of labour decreases total blood loss increases, but there is a poor correlation of uterine activity to total blood loss over the same period of time, probably because of biological variations in myometrial activity in normal women.

AND SummaryForty-four Chinese women of mixed parity had their energy output measured on one occasion; 28 in the first stage and 16 in the second stage of labour. No difference in energy output was noticed with varying parity. The energy output in the second stage of labour was found to be 40 per cent more than in the first stage. In another 39 women in different stages of labour who were not given sedatives, and 17 women who received 100 mg. pethidine in the second stage of labour, measurements of energy output, pulmonary ventilation, oxygen consumption and blood glucose were made during labour and after delivery. In women who were not given sedatives these values were found to be significantly higher in the second stage of labour than in the first stage and after delivery. The values were significantly lower in the sedated than in the unsedated women both during labour and after delivery. The blood glucose level was, however, found to be significantly higher in the sedated women than the unsedated women both during labour and after delivery.