Although yeast cannot normally incorporate exogenous deoxythymidine 5'monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sotl) that prevents strains carrying either tupi, tup2, or tup4 from growing on selective medium. The sotl mutation maps between radl and the centromere of chromosome XVI, and is unlinked to any of the tup mutations. The sotl mutation does not suppress the other pleiotropic effects of the tupl mutant, notably the lack of mating of tupl MATa strains. The sotl mutation specifically blocks the uptake of dTMP into tup strains. After growing a sotl strain in medium containing [3H]dTMP, we showed that the medium still contained more than 90% of the original [3H]dTMP and that this medium could support the incorporation of [3H]dTMP by a tup2 strain. Therefore, sotl strains do not degrade dTMP in the medium. The sotl mutation had no effect on the uptake of other nutrients essential for growth, including several amino acids, adenine, and uracil.
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