Summary. -This study investigated the anti-neoplastic potential of avian reovirus σC (sigma C) protein on Rous sarcoma virus-induced fibrosarcoma in chicken. The recombinant vector expressing σC protein was injected intra-tumorally into specific pathogen free chicken with fibro-sarcoma at the dose 100µg per bird, while control birds were mock-treated with 100µg of empty vector per bird. Recombinant σC protein induced apoptosis in tumors of treated birds resulting in progressive tumor regression, while similar changes were absent in tumors of mock-treated controls. The σC protein-induced apoptosis in tumors was quantified by flow cytometry and the mean level of apoptosis up to 66% was observed in treated tumors, whereas any significant level of apoptosis was absent in mock-treated controls.Keywords: avian reovirus; Rous sarcoma virus; tumors; σC protein; apoptosis E-mail: uma@kvasu.ac.in; phone: +91-487-2370344-275. Abbreviations: ARV(s) = avian reovirus(es); AO/EtBr = acridine orange/ethidium bromide; CEF = chicken embryonic fibroblast; H&E = hematoxylin and eosin; MRV = mammalian reovirus; MAb = monoclonal antibody; σC = sigma C, σ1 = sigma 1; SPF = specific pathogen free; RSV = Rous sarcoma virus
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.