Turnera subulata are bright common garden flowers that are grown in Asian regions. Mostly flowers of several kinds have medicinal properties and applications. This is as one of a kind that contains compounds that has medicinal application. The present studies are targeted to investigate the phytochemical composition through GC-MS technique, antimicrobial activity and antitumor activity via MTT assay against Hep G2 (or HepG2), a human liver cancer cell line. Ethanol (EtOH) extracts has reasonable antitumor activity against Hep G2 for period of 24 h and 48 h and the aqueous part was non-reactive. From gas chromatography‑mass spectrometry (GC-MS), a sum of 27 identified natural compounds exhibiting against cancer cell. Some traces of flavouring agents and antifungal agent with very low GC-MS peak lengths are too observed furaneol (2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one), benzeneacetaldehyde, benzoic acid and undecanoic acid. Hence, the result that indicates the flavouring agents including flavonoids along with phenolic and other acidic compounds have important characteristic property in reducing and treating against cancer cells.
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