Red blood cells (RBC) stored without plasma in a neomycin, low ionic strength
medium at 4 °C in excess of 24 h show alterations in antigen reactivity. There is a loss of
protease-sensitive RBC antigens and a protease-type increased IgG saline agglutinability of
Rh antigens that is associated with increased binding of 125 I anti-D. Both the serological
findings and the alteration in RBC membrane polypeptides are consistent with protease
modification of the membrane due to contamination of the RBC by leukocytes. Neomycin,
low ionic strength or leukocytes alone or in dual combination do not produce the observed
changes in antigen reactivity. The role of neomycin and low ionic strength in this phenomenon
and implication for quality control of reagent RBC used for antibody detection and
identification are discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.