The development of a 3-dimensional (3-D) navigation system of ferromagnetic particles in a flow system was performed. In order to improve the practice of using externally-applied magnetic fields for targeting the magnetic particles to a circumscribed body region, we tested the feasibility of a novel 3-D navigation system, made by applying a strong external (magnetic) field through a GdBaCuO bulk superconductor. A 3-D theoretical model is proposed and used in order to evaluate the efficiency of the navigation/retention of magnetic particles in the flow system. Furthermore, an experimental model system was made and the efficiency of a prototype system was examined. Comparisons of experimental and the corresponding calculation results were made to examine the theoretical model system.
Development of a simple method for converting the lipid envelope of an inactivated virus to a gene transfer vector was achieved a couple years ago in the medical school of Osaka University. Hemagglutinating virus of Japan (HVJ; Sendai virus) envelope (HVJ-E) vector was constructed by incorporating plasmid DNA into inactivated HVJ particles. This HVJ envelope vector introduced plasmid DNA efficiently and rapidly into various cell lines, including cancer cells and several types of primary cell culture. In the present study, efficiency of gene transfer was found to be greatly enhanced by application of a magnetic field. Therefore, we developed a new type of magnet for magnetically enhancing and targeting gene transfection system by using vectors associated with ferromagnetic particles coated with positively/negatively charged biopolymers, which can help to enhance and target gene delivery with higher efficiency. For the transfection experiment in vitro, the HVJ-E vector was mixed with ferromagnetic particles coated with biopolymer and this mixture was added to cultured cells which were set up under the permanent magnet. The effect of the dose of the ferromagnetic particles on the transfection efficiency was discussed. In order to clarify the effect of magnetic field gradient on the accumulation possibility of the magnetic particles and the accuracy of the targeted site in the blood vessels, calculation of the applied magnetic force for the ferromagnetic particles inside the blood vessel was also performed.
Cerium oxide used for glass polishing agent is processed as wastewater mixed with the polishing sludge of the glass. The recycling of cerium oxide may have a large influence on reduction of environmental burden and resources recycling. In the treatment process of wastewater, the complex of cerium oxide and iron flocculant is formed by co-precipitation to clear the fluid. In order to recycle the cerium oxide, it is necessary to eliminate the cohesion and then remove the iron content. In this study, the aggregated particles were dispersed by pH adjustment, and the iron content was separated by the developed high gradient magnetic separation system (HGMS) with superconducting magnet. The optimum condition was examined by calculation and experiment. In addition, we succeeded to remove the silica particle by magneto-Archimedes levitation. It was confirmed that ferrous particles and silica particles can be removed from wastewater by using superconducting bulk magnet, which makes it possible to reuse ceria particle as the polishing agent.Index Terms-Cerium oxide, glass polishing agent, HGMS, magnetic HTS bulk magnet, pH adjustment, silica.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.