Series IAfter anaesthesia with diethyl-ether one common carotid artery was ligated and transsected. Twenty-four hours later the animals were exposed to carbon monoxide (3000 ppm in room air) for 90 minutes while held in a plastic box with a volume of 3001, allowing the exposure of eight animals at a time. The gas mixture was stirred by a fan. The concentration of carbon monoxide was continuously monitored inside the plastic container and in its immediate vicinity by an infrared spectrometer (URAS I, Hartmann & Braun, FRG) to assure a continuous exposure of the animals and to protect laboratory personnel against accidental leaks. At the 48th hour after exposure the rats were anaesthetised with diethyl-ether and perfused via the ascending aorta with formalin after briefly washing out the vasculature with saline.
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