Ethnic value of many known plants are underexploited for medicinal application besides their proven traditional qualities. One such plant known for wound healing is Tridax procumbens. This plant has wound healing property and is commercially unexploited. Silver nanoparticle (Ag-NP) were synthesized using this plant extracts using different solvents (methanol, ethyl acetate and aqueous), which exhibit resonance at 426, 424 and 418 nm, respectively. This plant-mediated Ag-NPs have strong anti-bactericidal activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia, Serratia marcescens and Bacillus subtilis with methanol extract. Further instance, elemental composition was confirmed by energy dispersive X-ray analysis and particle size ranges were observed at 80-200 nm with spherical shape nanoparticles by scanning electron microscopy and transmission electron microscopy analysis. The biocompatibility of Ag-NPs was assessed using fibroblast cell line (L929) by MTT assay with 109.35 µg IC 50 value. The oxygen plasma treated and non-treated bamboo spunlaced nonwoven fabrics were coated with the Ag-NPs by exhaust method. Contact angle and water retention revealed significant difference in absorption ability of plasma treated fabric. Field emission scanning electron microscopy revealed the presence of Ag-NPs in plasma coated fabrics. The fabricated cloth was studied for anti-microbial and microbial penetration ability.
Probiotics yield numerous health benefits to the host. In this way the probiotic bacterium, Bacillus coagulans has competitively been excluded the pathogenic bacteria, Streptococcus spp., and Klebsiella spp., from the gut of Macrobrachium rosenbergii postlarvae when given through feed (Manjula et al., 2018). The elimination of these two pathogenic bacteria indicated the fact that B. coagulans improved the disease resistance capacity of M. rosenbergii. The present paper deals with 16S rRNA sequence analysis of gut microbial diversity of M. rosenbergii fed with B. coagulans (CFU, 2.28x10-7) supplemented feed, which revealed the presence of B. coagulans (1541 bp), Lactobacillus fermentum (1516 bp), Lactobacillus lactis (1499 bp), Lactobacillus acidophilus (1507 bp), Escherichia coli (1465 bp) and Staphylococcus aureus (1476 bp). The BLAST of these sequences showed almost 100% similarities with the same species retrieved from the NCBI database. The MAS showed 920 identical amino acids residues, 119 similar amino acids residues and 461 variable amino acids sites. The nucleotide sequence divergence and the calculated phylogenetic information clearly discriminate these bacterial species. Contribution/Originality: This study indicates that the probiotic, Bacillus coagulans used as a feed supplement helped in the exclusion of two pathogenic bacteria Streptococcus spp., and Klebsiella spp., from the gut of Macrobrachium rosenbergii. The gut microflora identified have been barcoded and authenticated.
In this study, Box–Behnken design was used to study the effect of optimization of plasma treatment on surface modification of bamboo spunlace nonwoven fabric. The effect and interaction of the process variables of glow discharge oxygen plasma treatment, i.e. discharge power, exposure duration and inter-electrode spacing on fabric characteristics such as mass per unit area, thickness, breaking force and elongation, air permeability, water vapour permeability and wicking were analysed. The optimum conditions of discharge power 650 W, exposure duration 55 s, and inter-electrode spacing 4 cm were arrived using response optimizer tool of Minitab software. The fabrics showed better characteristics in terms of air permeability, water vapour permeability, wicking and elongation and showed slight reduction in terms of fabric weight, thickness and breaking force. Fourier-transform infrared spectroscopy spectra analysis confirmed the increased functionality of the material after plasma treatment.
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