A conclusive study was conducted for preparation and evaluation of combined inactivated entero-4 vaccine containing bovine rotavirus (BRV), bovine coronavirus (BCV), E. coli K 99 and toxoid of C. perfringens type "C".Laboratory and field evaluations were conducted on laboratory animals, calves and late pregnant cows with monitoring the active and passive antibodies in vaccinated cows and their offspring respectively. Laboratory evaluation proved purity, safety and high efficacy of the vaccine without interference between different vaccine ingredients. Field evaluation gave satisfactory results when pregnant cows vaccinated at late stage of pregnancy with high neutralizing antibody titers against BRV, BCV and C. perfringens as well as high E. coli agglutinating titers. Maternal immunity passively protected offspring during the critical period of age and remained protected till the end of sampling time (30 th day post parturition).
Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as from Enterotoxigenic E. coli strain K99. Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including: microagglutination test, indirect ELISA and immunofluorescent antibody technique. Results of this endeavor were correlated to that of the standard virus neutralization test. The locally prepared antigens were proved useful for vaccine evaluation. Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E. coli.
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