Soil salinity is one of the constraints of crop production in Egypt. The aims of this study were to identify genomic regions associated with grain weight and its related traits along with their salinity tolerance indices and to identify the most salinity tolerant and high-yielding genotypes. Therefore, we evaluated an advanced backcross mapping population of barley in newly reclaimed soil under two salinity levels of groundwater aquifers in South of Sinai, Egypt. We detected significant QTL associated with grain weight related attributes and the salinity tolerance index (STI) distributed throughout the whole genome of barley, which can be used to enhance salinity tolerance. Moreover, the markers bPb-3739 (4H, 96.3 cM), AF043094A (5H, 156 cM), bPb-8161 (7H, 2.22 cM), and bPb-5260 (7H, 115.6 cM), were the most important identified genomic regions corresponding to vernalization, dwarfing and dehydrin genes, which are correlated with salinity tolerance. Additionally, the doubled haploid lines SI001, SI043, SI044, SI028, SI242, SI035, and SI005 had the highest STI values based on yield average. The present study demonstrated that wild and elite barley do harbor novel valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits under salinity conditions.
While previous studies have demonstrated the positive effects of low cobalt (CB) levels and chitosan (CH) on yield and nutrient status, information about their individual and combined applications on plants under stress is still lacking. Therefore, we conducted a study to investigate CB and CH impacts on mitigating water stress during growth stages and their effects on corn macronutrient uptake and yield. Four irrigation schemes were employed, including (1) control (full irrigation), (2) 70% of irrigation water during the vegetative stage, (3) 70% of irrigation water during the flowering stage, and (4) 85% of irrigation water during both the vegetative and flowering stages. The plants were treated with (7.5 mg l−1) CB injected into the irrigation water and CH foliar application (500 mg l−1), while distilled water was used as the control. Plants that were exposed to water stress during the flowering stage and treated with CB, or those subjected to water stress during the vegetative stage and treated with CH, showed increased macronutrient uptake and growth, which had a positive effect on yield and water use efficiency. However, when CB and CH were applied in combination, their potential to enhance these features depended on the pattern of water stress adopted. Overall, the application of CB and CH was effective in mitigating water stress, and their combined application was particularly effective when 70% of irrigation water was applied during the flowering stage. This approach resulted in the highest yield, macronutrient uptake, water use efficiency, and tolerance index.
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