The Rabies – a viral disease characterized by severe damage of the nervous system. The analysis of data from the Rospotrebnadzor for 4 years (from 2015 to 2018) on the varmint of animals with rabies in four regions of the Central Federal District with different population densities is carried out. The main carriers of rabies virus in the region are the red fox, raccoon dog, stray dogs and cats; the role of other species is not so significant and rarely exceeds 1% of the total number of animals with suspected rabies. It has been established that in areas with a low population (Tver region) the role of a raccoon dog in rabies transfer can be even more significant than red foxes. To reduce the incidence of human rabies, constant monitoring and regulation of the numbers of foxes and raccoon dogs in the wild, as well as stray dogs and cats, is highly required. Consequently, we consider the current tendency to remove stray dogs from their habitats, their sterilization and return to urbocenosis insufficient; sterilized dogs will remain a source of rabies in populated locations.
In experiments to infect aquatic invertebrates in the zooculture, we used influenza A viruses, namely, to infect crustaceans Daphnia magna Straus, 1826 – human influenza virus, Hong Kong strain 1569/79 (H3N2), and to infect molluscs Anodonta cygnea Linné, 1758 – influenza virus A birds, Strain Rostok 1/34 (Hav1Neq1) – the so-called true bird plague virus. As a result of a series of experiments, found that influenza A viruses persist in the water for no more than 3 days, while in the gills and mantle of molluscs the virus is isolated on chicken embryos for at least another 35 days after contact with virus-containing water (a total of 70 individuals were studied). From the body Daphnia magna, to isolate the human influenza A virus on chicken embryos was possible within 14 days after infection through water (examined 6,800 individuals), by the method of immunofluorescence the influenza virus was determined in the intestines of crustaceans during the entire period of observation – 70 days from the time of infection. Influenza A viruses do not have a harmful effect on crustaceans or molluscs, infected animals also develop and reproduce, as well as individuals of control groups. Interesting is the fact that we have established the possibility of the loss of agglutination of red blood cells of chickens as a result of the reproduction of the human influenza A virus in the body of invertebrate Daphnia magna, which indicates a change in the viral protein hemagglutinin. The use of aquatic invertebrate zooculture can help in the study of the circulation of influenza A viruses in nature, as well as in the study of the variability of influenza A viruses.
Potentialities of the flow cytometry method in studies of the qualitative and quantitative composition of endometrial immunocompetent cells are demonstrated. The studied leukocyte populations are found to occur in negligible quantities in the endometrium.
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