Objective: To investigate the antidiabetic effect of aqueous extract of Chlorophytum borivilianum (safed musli) root. Methods: The collected roots were washed; shade dried and was pulverized with a mechanical pulverizer for the size reduction. It was then passed through mesh of size # 60 and the fine powder was collected and was used for the experiment for powder microscopy and preparation of extract. The root powder (1000 g) was subjected to cold maceration in 2 L of sterile distilled water for 48 hours at room temperature, filtered into a clean round bottom flask. The α-Glucosidase inhibitory activity was conducted where 100µl of a sample of different concentrations was incubated with 50µl α-glucosidase (1.0 U/ml) (from Saccharomyces cerevisiae) in phosphate buffer (0.1 M, pH 6.8) for 10 min at 37°C. The reaction was initiated by addition of 50 µl of substrate: 5 mM, p-nitrophenyl-α-D glucopyranoside in a 0.1 M phosphate buffer at pH 6.8. P-nitrophenol's release kinetics. The α-Amylase Inhibition where 250µL of the extract (5mg/mL) was preincubated with 250µL of-amylase solution for 10min at 25°C in one set of tubes. In another set of tubesamylase was preincubated with 250µL of phosphate buffer (pH 6.9). 250µL of starch solution at increasing concentrations (0.30-5.0mg/mL) was added to both sets of reaction mixtures to start the reaction. The mixture was then incubated for 10min at 25°C and then boiled for 5min after the addition of 500µL of DNS to stop the reaction. Results: In this study acarbose was also used as a standard drug for α-glucosidase inhibitor. Acarbose at a concentration of (100-1000 µg/ml) showed αglucosidase inhibitory activity from 30. 31±0.1084to 84.05±0.2075% with an IC value 411.436567µg dry extract, & acarbose was also used as a standard drug for α amylase inhibitor. Acarbose at a concentration of (100-1000 µg/ml) showed α-glucosidase inhibitory activity from 23.33±0.1415 to 62.05±0.2725% with an IC value 236.774194µg dry extract. Conclusions: As a result, we found that the extract of C. boriviliamum have free radical scavenging activity and inhibitory activity against α-amylase and α-glucosidase and this therapeutic potentiality could be exploited in the management of post prandial hyperglycemia in the treatment of type 2 diabetes mellitus.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.