Structure determination of biomacromolecules under in-cell conditions is a relevant yet challenging task. Electron paramagnetic resonance (EPR) distance measurements in combination with site-directed spin labeling (SDSL) are a valuable tool in this endeavor but the usually used nitroxide spin labels are not well-suited for in-cell measurements. In contrast, triarylmethyl (trityl) radicals are highly persistent, exhibit a long relaxation time and a narrow spectral width. Here, the synthesis of a versatile collection of trityl spin labels and their application in in vitro and in-cell trityl-iron distance measurements on a cytochrome P450 protein are described. The trityl labels show similar labeling efficiencies and better signal-to-noise ratios (SNR) as compared to the popular methanethiosulfonate spin label (MTSSL) and enabled a successful in-cell measurement.

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Development of high‐performing lithium‐based batteries inevitably calls for a profound understanding and elucidation of the reactivity at the electrode–liquid electrolyte interface and its impact on the overall performance and safety. The formation, composition, properties, and mechanisms of the cathode electrolyte interphase (CEI) formation and function are still to a large extent unknown for most lithium‐based battery materials, whereas the same is well considered for the solid electrolyte interphase on negative electrodes in the literature. In particular, in high voltage regions >4.3 V, the oxidative stability limit of most liquid electrolytes is reached and new mechanisms, involving surface reactivity of the active material beside electrolyte decomposition, contribute to the interfacial reactivity and nature of the CEI. Focusing on lithium‐based cell chemistries, this review aims to highlight the impact of the still less understood electrolyte decomposition chemistry, dictated by the nature of its components, as well as the in‐depth research on the physicochemical and electrochemical properties of CEI formation and evolution at positive electrode material surface and sub‐surfaces.

Structure determination of biomacromolecules under in-cell conditions is ar elevant yet challenging task. Electron paramagnetic resonance (EPR) distance measurements in combination with site-directed spin labeling (SDSL) are av aluable tool in this endeavor but the usually used nitroxide spin labels are not well-suited for in-cell measurements.I nc ontrast, triarylmethyl (trityl) radicals are highly persistent, exhibit along relaxation time and anarrowspectral width. Here,the synthesis of aversatile collection of trityl spin labels and their application in in vitro and in-cell trityl-iron distance measurements on ac ytochrome P450 protein are described. The trityl labels shows imilar labeling efficiencies and better signal-to-noise ratios (SNR) as compared to the popular methanethiosulfonate spin label (MTSSL) and enabled asuccessful in-cell measurement.