A collective infraGred laser scattering diagnostic has been installed on the TORE SUPRA tokamak for the measurement of plasma density fluctuations. For the range of wavenumbers explored (3-15cm-'), the scattering angles are very weak (-1 mrad). Consequently, the scattering signals are averaged along the whole observation chord, resulting in poor longitudinal spatial localization. However, by virtue of the pitch angle variation of the magnetic field lines in the tokamak, and of the perpendicularity of the turbulence wavevector to these field lines, it has been possible to obtain partial spatial resolution along the direction of the beam. Good agreement between the experimental and theoretical angular resolution of the diagnostic as well as the results of cross-correlation performed on the signals obtained by two simultaneous probing beams also justiiy this novel concept.From the variation of the fluctuation power with the orientation angle of the observed wavevector, it has been possible to deduce the radial fluctuation profile by a deconvolution procedure, showing that the fluctuations increase sharply near the edge.The k-spectrum was also measured and shows a k-3 dependence for k>6cm-'. Experimental evidences are put foward to show that the k-spectrum is neither purely poloidal nor purely radial in the (k,k,) plane.

A collective laser light scattering diagnostic ALTAIR (a french acronym for local analysis of anomalous transport using infrared light), using a CO2 laser beam (λ=10.6 μm) has been realized to measure plasma density fluctuations in the TORE SUPRA tokamak. This article describes in detail the optical setup, the signal processing, acquisition, and control systems required for this experiment. As the density fluctuations propagating in tokamaks have small wave numbers and require small scattering angles, such scattering experiments are considered as having no resolution along the beam. However, taking advantage of the pitch angle variation of the magnetic field lines around the magnetic axis along a vertical chord, it has been possible to obtain partial spatial localization of the scattering volume by rotating the direction of the analyzed wave vector in a horizontal plane. Heterodyne detection is used to determine the fluctuations propagation direction. The experiment has been tested on acoustic waves and the first results obtained on TORE SUPRA indeed show the existence of a spatial resolution.

Transient receptor potential channel subfamily A member 1 (TRPA1) is a non-selective cationic channel, identified initially as a cold sensory receptor. TRPA1 responds to diverse exogenous and endogenous stimuli associated with pain and inflammation. However, the information on the role of TRPA1 toward T-cell responses remains scanty. In silico data suggest that TRPA1 can play an important role in the T-cell activation process. In this work, we explored the endogenous expression of TRPA1 and its function in T cells. By reverse transcription polymerase chain reaction (RT-PCR), confocal microscopy and flow cytometry, we demonstrated that TRPA1 is endogenously expressed in primary murine splenic T cells as well as in primary human T cells. TRPA1 is primarily located at the cell surface. TRPA1-specific activator namely allyl isothiocyanate (AITC) increases intracellular calcium ion (Ca2+) levels while two different inhibitors namely A-967079 as well as HC-030031 reduce intracellular Ca2+ levels in T cells; TRPA1 inhibition also reduces TCR-mediated calcium influx. TRPA1 expression was found to be increased during αCD3/αCD28 (TCR) or Concanavalin A (ConA)-driven stimulation in T cells. TRPA1-specific inhibitor treatment prevented induction of cluster of differentiation 25 (CD25), cluster of differentiation 69 (CD69) in ConA/TCR stimulated T cells and secretion of cytokines like tumor necrosis factor (TNF), interferon γ (IFN-γ), and interleukin 2 (IL-2) suggesting that endogenous activity of TRPA1 may be involved in T-cell activation. Collectively these results may have implication in T cell-mediated responses and indicate possible role of TRPA1 in immunological disorders.

Results on confinement and turbulence from a set of Ohmic discharges in TORE SUPRA are discussed. Attention is focused on the saturation of the energy confinement time and it is emphasized that this saturation can be explained by a saturation of the electron heat diffusivity. The ion behaviour is indeed governed by dilution and equipartition effects. Although the ion heat transport is never neoclassical, there is no enhanced degradation at saturation. This behaviour is confirmed by turbulence measurements using CO2 laser coherent scattering. The level of density fluctuations follows the electron heat diffusivity variations with the average density. Waves propagating in the ion diamagnetic direction are always present in turbulence frequency spectra. Thus, the saturation cannot be explained by the onset of an ion turbulence. The existence of ion turbulence in the edge at all densities cannot be excluded. However, this ion feature in scattering spectra could be explained by a Doppler shift associated with an inversion point of the radial electric field at the edge

(2013) Thermosensitive ion channel TRPV1 is endogenously expressed in the sperm of a fresh water teleost fish (Labeorohita) and regulates sperm motility, Channels, 7:6, 483-492,

Two-dimensional (2-D) density and potential measurements have been carried out for plasma diffusing through an aperture in a diverging magnetic field. The radial density profile near the source is peaked on the axis but gradually evolves into a hollow profile away from the source. We observe a slow increase of the peak density along a hollow conical surface and correlate with the 2-D potential measurement reported earlier. It is also shown that the formation of 2-D structures with similar features are observed whenever plasma is allowed to diffuse through a physical aperture in such diverging magnetic field configuration, with or without the presence of electric double layer, i.e., the phenomenon is generic in nature.

TRPV1 is a thermo-sensitive ion channel involved in neurosensory and other physiological functions. The trans-membrane helices of TRPV1 undergo quick and complex conformational changes governed by thermodynamic parameters and membrane components leading to channel opening. However, the molecular mechanisms underlying such events are poorly understood. Here we analysed the molecular evolution of TRPV1 at the lipid-water-interface region (LWI), typically defined as a layer of 6 Å thickness on each side of the membrane with less availability of free water. Amino acids demarcating the end of the trans-membrane helices are highly conserved. Residues present in the inner leaflet are more conserved and have been preferentially selected over others. Amino acids with snorkeling properties (Arginine and Tyrosine) undergo specific selection during the vertebrate evolution in a cholesterol-dependent and/or body temperature manner. Results suggest that H-bond formation between the OH- group of cholesterol and side chain of Arg557 or Arg575 at the inner leaflet is a critical parameter that can regulate channel functions. Different LWI mutants of TRPV1 have altered membrane localization and deficient colocalization with lipid raft markers. These findings may help to understand the lipid-protein interactions, and molecular basis of different neuronal functions. Such findings may have broad importance in the context of differential sensory responses, pathophysiologies, and application of pharmacological drugs such as anaesthetics acting on TRPVs.

In a RF produced magnetized argon plasma expanding into a larger expansion chamber, electrostatic modes propagating azimuthally in the direction of the electron diamagnetic drift and frequency greater than the ion cyclotron frequency are observed. In the radial direction, the mode amplitude peaks at a location where the radial density gradient is maximum. The modes are detected at axial locations up to 16 cm away from the entrance aperture. For fixed values of the neutral pressure and magnetic field, the mode frequency is found to be independent of the location at which it is measured. The modes exhibit drift wave characteristics revealing a radial structure with the azimuthal mode number m = 1 at the lower radial locations (r ∼ 3.0 cm) while the m = 2 mode is located in the outer region. Theoretical modeling using a local dispersion relation based on the fluid equations predicts destabilization of the modes with frequency greater than the ion-cyclotron frequency by electron-neutral collisions and exhibiting other drift wave features.