The aim of the experiment was to study the changes in the activities of various rumen fibre degrading enzymes due to the feeding of chemically treated mustard (Brassica campestris) straw in sheep. Mustard straw (MS) (<5 cm particle size) was treated either with urea (4% (w/w), or with 2% sodium hydroxide (NaOH), or with alkaline hydrogen peroxide (2% NaOH and 1.5% hydrogen peroxide (H 2 O 2 )) and/or supplemented with 2% (w/w) urea. Seven maintenance type rations were prepared using MS (70 parts) with molasses (5 parts) and concentrate (25 parts). They were untreated MS (CMS), urea treated MS (UMS), urea supplemented MS (MS-US), alkali treated MS (AMS), alkali treated and urea supplemented MS (AMS-US), alkali H 2 O 2 treated MS (AHMS) and alkali H 2 O 2 treated and urea supplemented MS (AHMS-US). They were then compressed into a complete feed block with the help of block making machine. Forty two male hoggets of Malpura breed sheep were equally distributed into each treatment group and (were) offered feed and water ad libitum. At the end of 21 days of feeding trial, rumen liquor was collected through stomach tube from three animals in each group at 0 h, 4 h, 8 h, 12 h of post feeding. Results showed that the level of enzyme varied from 8.52 to 11.12, 40.85 to 50.37, 3.22 to 3.78, 2.09 to 2.77 and 31.44 to 44.24 units/100 ml SRL respectively for carboxymethyl cellulase (CMCase), α-amylase, microcrystalline cellulase (MCCase), filter paper (FP) degrading enzyme and α-glucosidase. Processing of MS affected the enzyme activities, in a way, that NaOH and AHP treatment significantly reduced CMCase and FP degrading enzyme. The effect of urea treatment showed an increase in the activity of MCCase and α-glucosidase. But the supplementation of urea increased the activity of CMCase, FP degrading enzyme and α-glucosidase. The CMCase, α-amylase, α-glucosidase activities were highest at 4hr whereas MCCase and FP degrading enzyme had maximum activities at 12 h post feeding Results suggested that MS might need longer time in the rumen for its effective degradation.
The present study was carried out to evaluate the influence of dietary supplementation of probiotic bacteria (Lactobacillus acidophilus), on growth performance of Labeo rohita. The probiotic L. acidophilus was procured from the market and the feed was prepared by crushing the basal food with probiotic bacteria in different combinations. The feeding trials were conducted in triplicate for 60 days, to determine the effect of dietary probiotics on growth status of fish. For this, the live fishes (L. rohita) were captured from Sagar Taal, Budaun, U.P. After acclimatization for 10 days, the fishes with similar body weight were distributed randomly into five treatment groups at
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