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The technique of irreversible electroporation has been successfully applied to cause a lethal effect on Lactobacillus brevis cells suspended in phosphate buffer solution, Na(2)HPO(4)/NaH(2)PO(4) . H(2)O (0.845/0.186 mM) between parallel plane electrodes. Tests were carried out at different temperatures (24,45,60, and 80 degrees C) to determine if there was a synergistic effect of temperature and electric pulse treatment on the destruction of L. brevis. Experimental results indicate that the viability (log N/N(0); where N(0) and N are the number of cells survived per milliliter before and after pulse voltage application, respectively) of L. brevis decreased with electric field strength E and temperature T and treatment time t(t). The relations between log(N/N(0)) and t(t) and log(N/N(0)) and E indicate that higher field strengths are more effective than higher treatment times in causing destruction of L. brevis cells. It was also found that as the temperature of the liquid medium containing L. brevis cells increased from 24 to 60 degrees C, the death rate of L. brevis cells increased with a decrease in the total treatment time t(t) (pulse width x number of pulses applied). The application of an electric field strength E = 25 kV/cm at 60 degrees C and treatment time t(t) = 10 ms resulted in very high destruction levels of L. brevis cells (N/N(0) = 10(-9)). In comparison with existing steam sterilization technology, this new method of sterilization using relatively low temperature and short treatment time could prove to be an excellent method to minimize thermal denaturation of important nutrient components in liquid media.

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The paper presents the results of using the fundamental and the low frequency harmonic components of leakage current to study aging of silicone ruhher in saltfog. Experiments have been conducted on RTV and HTV coated rods at different fields (0.25-0.6 kV/cm) and conductivities (1000 to 2500 pS/cm). The onset of dry-hand arcing on samples could he determined hy measuring the low frequency harmonic components. A correlation has been found between the fundamental and low harmonic components of leakage current and different forms of aging. Where erosion could be associated with an increase in the level of both the fundamental and low frequency harmonic components of leakage current. For example, surface damage for HTV rods occurred when the fundamental component of leakage current was greater than 2 mA. On the other hand, when the samples approached failure, the fundamental component of leakage current reached relatively high values ( > 6 mA for HTV rods and > 2 mA for RTV rods) and the low frequency harmonic components of the leakage current tended to decrease. The results suggest that both the fundamental and low frequency harmonics of leakage current can be used as a tool to determine both the beginning of aging and end of life of silicone rubher in salt-fog.

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