67P et al., 1970) that atractyloside and trialkyltins act at different sites. The ATPase of broken mitochondria is inhibited by oligomycin, unlike the solubilized ATPase (Selwyn & Chappell, 1962). The soluble ATPase from acetone-dried ox heart mitochondria (Selwyn, 1967) is not inhibited by trimethyltin at concentrations up to 250-M. Kagawa & Racker (1966) have shown that the soluble ATPase is inhibited by tributyltin only in the presence of the factor that confers oligomycin-sensitivity on the ATPase. These observations leave little doubt that the site of action of trialkyltins is the same as that of oligomycin.
It has been found that locust flight muscle mitochondria will accumulate calcium ions (in the presence of phosphate) rapidly and stoichiometrically from a sucrose or Tris‐Cl medium, but only slowly from a potassium chloride medium.
The mitochondria are capable of accumulating small amounts of calcium ions (100 ng ions/mg protein) in the absence of any permeant anions. Acetate will not support massive accumulation.
Calcium uptake is blocked by low concentrations of praeseodymium ions.
Passive swelling experiments in solutions of calcium salts indicate that the mechanism of calcium ion entry into the mitochondria is similar to that observed for rat liver mitochondria.
The mitochondria were found to be very permeable to the anions chloride and isethionate.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.