Enhanced expression of the human ferritin H- and L-chain genes (hfH and hfL) was achieved in Saccharomyces cerevisiae by modifying the N-terminal region of the structural genes. The yeast episomal vector YEp352 with the galactokinase1 (GAL1) promoter was used to construct expression plasmids. The expression of each gene was examined using SDS-PAGE and Western blot analysis. Iron uptake was examined and the cellular iron concentration was increased in S. cerevisiae expressing hfH. When cultured cells were incubated with 14.3 mM Fe(2+), the recombinant yeast expressing hfH had a cellular iron concentration 1.5 times greater than that of the control strain. The relationship between the iron taken up by the cells and the expressed proteins was examined. Iron-binding H-chain ferritin (H-ferritin) was seen in the recombinant S. cerevisiae incubated with iron, while small amounts of iron-binding L-chain ferritin (L-ferritin) were observed. Combined, these observations demonstrate that human H-ferritin has a function in iron storage in S. cerevisiae, while L-ferritin does not.
The ESPI (Electronic Speckle PatternInterferometry) is a real-time, full-field, nondestructiveoptical measurement technique. In thisstudy, ESPI was proposed for the purpose ofvibration analysis for new and composite materials.Composite materials have various complicatedcharacteristics according to the materials,orientations, and the stacking sequences of the plyand boundary conditions. Therefore, it is difficult toanalyze composite materials. For efficient use ofcomposite materials in engineering applications thedynamic behavior (i.e., natural frequencies andnodal patterns) should be known. With the use ofTime-Average ESPI, one could easily analyzevibration characteristics of composite material byreal time. We manufactured two kinds of laminatedcomposites (i.e., symmetry and asymmetry) whichwere composed of CFRP(Carbon Fiber ReinforcedPlastics) and the shape of the test piece was ofrectangular form.
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