Due to their ultra-short duration and peak currents in the kA range 1,2 , laserwakefield accelerated electron bunches are promising drivers for ultrafast X-ray generation in compact free-electron-lasers (FELs), Thomson-scattering or betatron sources [3][4][5] . Here we present the first single-shot, high-resolution measurements of the longitudinal bunch profile obtained without prior assumptions about the bunch shape. Our method allows complex features, such as multi-bunch structures, to be detected. Varying the length of the gas target, and thus the acceleration length, enables an assessment of the bunch profile evolution during the acceleration process. We find a minimum bunch duration of 4.2 fs (full width at half maximum) with shot-to-shot fluctuation of 11% rms. Our results suggest that after depletion of the laser energy, a transition from a laser-driven to a particle-driven wakefield occurs, associated with the injection of a secondary bunch. The resulting double-bunch structure might act as an elegant approach for driver-witness type experiments, i.e. allowing a non-dephasinglimited acceleration of the secondary bunch in a plasma-afterburner stage 6,7 .Since the first demonstration of high-quality, quasi-monochromatic electron beams in 2004, laser wakefield acceleration (LWFA) has become a reliable scheme to accelerate electrons bunches to energies in the GeV range in plasma accelerator stages a few cm long 8-12 . The small scale of the acceleration structure, confining the bunch to a fraction of the plasma wavelength, implies bunch durations in the femtosecond range.Determining the detailed longitudinal profile of the generated bunches is important for understanding the accelerator dynamics, enabling accelerator control, and for determining their potential applications, such as driving compact FELs 13,14 . However, the limited temporal resolution of traditional methods, such as electro-optic sampling 15 , prevents their application to measuring the ultra-short bunches produced by an LWFA.Although recent experiments confirmed the ultra-short nature of LWFA electron beams 1,2 these relied on the assumption of a Gaussian longitudinal profile when determining the electron bunch duration. As in earlier work 2 , we determine the bunch profile from measurements of the spectrum of coherent transition radiation (CTR).However, our experiments advance prior work in several key aspects: (i) the bandwidth of the recorded spectrum covers a spectral range of more than 4 octaves at high resolution; (ii) the spectrum was recorded in a single-shot, preventing shot-to shot fluctuations in the electron bunch parameters distorting the measured spectrum; (iii) the