Polyaniline-Na + -montmorillonite nanocomposite particles were synthesized using an emulsion intercalation method, and electrorheological (ER) fluids were produced by dispersing the synthesized nanocomposite particles in an electrically-insulating silicone oil. The emulsion of an aniline monomer with dodecyl benzenesulfonic acid was inserted into the layers of clay, and polymerization was processed by adding the oxidant initiator solution. DBSA as a emulsifier and a dopant took a important role for polyaniline clay nanocomposite. This insertion of polyaniline was confirmed by Xray diffraction. To observe its ER properties, we measured the shear viscosity and the shear stress by controlling shear rate. Furthermore, we conducted dynamic tests to investigate the viscoelastic properties of the ER fluid under an electric field in the linear viscoelastic region.
Collagen is the most abundant protein found in the extracellular matrix and is widely used to build scaffolds for biomedical applications which are the result of its biocompatibility and biodegradability. In the present study, we constructed a rhOCN/FNIII9-10 fusion protein and rhOCN/FNIII9-10-functionalized collagen matrices and investigated the potential value for bone tissue engineering. In vitro studies carried out with preosteoblastic MC3T3-E1 cells showed that rhOCN/FNIII9-10 fusion protein promoted cell adhesion and the mRNA levels of osteogenic markers including osteocalcin, runt-related transcription factor 2, alkaline phosphatase (ALP), and collagen type I. In addition, rhOCN/FNIII9-10-functionalized collagen matrices showed significant induction of the ALP activity more than rhFNIII9-10-functionalized collagen matrices or collagen matrices alone. These results suggested that rhOCN/FNIII9-10-functionalized collagen matrices have potential for bone tissue engineering.
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