This study was carried out at Mehallet Mousa Animal production Research station, Animal production Research institute- ministry of agriculture, Egypt. This work was carried out on ten late pregnant buffaloes for studying prostaglandin (PG F2α) during pre and post partum periods. Blood samples were collected five days prepartum and one week postpartum. Prostaglandin was determined by Elisa (Enzyme-Limked immunosorbant Assay). The results, showed that the plasma prostaglandin levels on day three, two and one prepartum was higher than on day five prepartum. On The day of delivery (0 day) a sudden sharp increase in PGF2& con concentration occurred (180.83±4.23 pg/ml) followed by a gradual decrease in the plasma concentration. Of PGF2α on day four, five, six and seven postpartum, small respectively