This paper investigates the cultural-morphological, physiological and biochemical properties of bacterial isolates with stimulating properties isolated from the rhizosphere of cotton (Gossýpium hirsútum) grown in saline soils of Uzbekistan. The cells of the isolated culture No. 12 were found to be rod-shaped, 2-3x0.5-0.6 urn in size, single or chain-linked, Gram-positive aerobic bacteria producing spores. In studying the culture No. 146, its cells were in the form of thick, less mobile rods, 1-2x0.6-0.8 urn in size, forming thermostable spores. The spores were located in the center of the cell; the Gram-stained colonies in meat-peptone agar were round, bulging; the edges were flat, consistency oily, smooth and, mucous; and, the upper part was found to be shiny. Studies have shown that the isolates appertained to the genus Bacillus sr.
In this article, a microbial inoculant acting as a biofertilizer is considered as a biostimulant, consisting of three strains of local salt-tolerant strains of rhizobacteria Pseudomonas stutzeri SKB308, Bacillus subtilis SKB309, Bacillus megaterium SKB310. The authors have developed and introduced into agricultural practice an experimental sample of the biological product ‘Zamin-M’, which increases the resistance of cotton to stress conditions of soil salinity. It is proved that the biological product ‘Zamin-M’ increases soil fertility has a beneficial effect on the enzymatic activity of the soil and the development of a population of beneficial microorganisms. The bioproduct ‘Zamin-M’ is presented as a new stimulant in the practice of growing cotton in saline conditions. An agricultural technique for the use of a local biological product in agriculture has been developed. The ‘Zamin-M’ is included by the State Committee of the Republic of Uzbekistan in the list of agrochemicals and pesticides permitted for use in agriculture of the Republic of Uzbekistan (certificate No. 1A1005).
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