The Guangxi yellow-feather chicken is a very important breed used as a broiler in southern China, but the pure line is being threatened by continual introduction of foreign genetics into its breeding program to make it more marketable. In the current study, we isolated primordial germ cells (PGCs), a cell type committed to form sperm or eggs and that is responsible for passing genetic material from one generation to the next, from Guangxi yellow-feather chickens and cultured them in a cell-insert system. Three stable cell lines, all male, were established from 10 isolations. These cells proliferated and expressed germ cell-related markers such as SSEA-1 and EMA-1 after prolonged culture in vitro. After genetic modification, these PGCs retained significant potential to colonize the gonads and give rise to gametes when they were reintroduced into the vasculature of stage-15 HH embryos, confirming their germline cell characteristics. The ability to culture PGCs and preserve the genetics from this species would not only be of significant importance for biodiversity conservation, but also holds promise for use of these cells in breeding strategies in the future.
Fabric-phase sorptive extraction (FPSE) coats organic-inorganic hybrid sorbent materials onto flexible and hydrophilic fabrics through sol-gel sorbent-coating technology. Herein, we explored four different coating chemistries, including those of nonpolar sol-gel poly(dimethylsiloxane), medium polar sol-gel poly(tetrahydrofuran), and polar sol-gel poly(ethylene glycol) (PEG)-block-poly(propylene glycol)-PEG, and sol-gel PEG. Results showed that PEG had the best extraction recoveries for the majority of the analytes. Subsequently, strongly polar sol-gels were prepared using PEG as precursor and FPSE medium. FPSE was then combined with a cut solid-phase extraction column and immobilised with magnetic-stirring beads so that FPSE could be automatically stirred magnetically throughout the entire extraction process. The developed method based on ultra-high-performance liquid chromatography-tandem mass spectrometry showed good linearity within the concentration range of 5-200 ng/mL. Under the optimum experimental conditions, limits of detection for 48 pesticides were obtained within the range of 0.014-6.280 ng/mL. Thereafter, the new method was applied to tomato, cucumber, and cabbage samples. Recoveries ranged from 71.4 to 99.8% at three concentration levels. The various parameters of this experimental method were systematically investigated and optimised to improve its sensitivity and prove its feasibility in vegetables. Overall, our results demonstrated that the method was simple, sensitive, efficient, and rapid. Furthermore, FPSE fibre cloth showed great potential for commercial exploitation because of its reusability.
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