The aim of the study was to examine the effect of a new tripeptide, Leu-Ile-Lys, on an experimental indometacin-induced gastric ulcer in rats. Materials and Methods: The experiment was performed with 24 male Wistar rats (average weight of 150 g). Rats were randomly divided into 3 groups: Group 1 (n=8)-the ulcer control group (IIGU), Group 2 (n=8)-the experimental group (IIGU+pretreating with the tripeptide Leu-Ile-Lys), and Group 3 (n=8)-the comparison group (IIGU+pre-treating with omeprazole). The model of IIGU in rats was performed by a single intragastric administration of indomethacin (60 mg/kg in 1ml of physiological saline). In Group 1, indomethacin caused the appearance of severe injuries of the mucosa with the presence of extensive edema and leukocyte infiltration in the submucosal layer. In animals of Group 2, which were pre-treated with the tripeptide Leu-Ile-Lys, macroscopically gastric mucosa also looked smooth and atrophic changes were not found. Destructive changes were not severe; they appeared only in the form of small spot erosions. The number of spot erosions was 2.6 times less than in Group 1. The average erosion depth was 6.8 times less than in Group 1, and 2.0 times less than in Group 3. Conclusion: Results of this study demonstrated the high, comparable to the action of omeprazole, gastroprotective activity of the new tripeptide Leu-Ile-Lys.
This article presents the results of a study aimed at determining the gastroprotective activity of a number of short-chain peptides against the background of experimental gastropathy in rats. Indomethacin ulcer was used as a model of ulceration. Omeprazole, a drug of basic therapy, was used as a comparison drug. According to the results of the study, two peptides — tetrapeptide LA-4 and hexapeptide LF-6 — demonstrated the gastroprotective activity. The effect was manifested in a signifi cant decrease in the average number of erosions on the surface of the gastric mucosa, their depth, the number of stripe-shaped erosions and Paul’s Index. At the same time, the LF-6 peptide had an effect comparable to that of omeprazole, which makes it promising and interesting for further study.
Aim. To determine the effect of Leu-Ile-Lys tripeptide on indicators of oxidative stress and expression of cyclooxygenase-1 and -2 in the gastric mucosa on the background of experimental indomethacin-induced ulcer. Methods. Experiments were performed on 35 male Wistar rats with a body mass of 200-250 g aged 2-3 months that were divided into 3 groups: the control group (intact rats, 8 animals), control group (simulation of indomethacin-induced gastropathy, 12 animals) experimental group (simulation of indomethacin-induced gastropathy + administration of Leu-Ile-Lys tripeptide, 15 animals). The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity at least 98%) was administered intragastrically daily for 7 days before the simulation of indomethacin-induced damage of the gastric mucosa in a dose of 11.5 mg/kg. The model of indomethacin-induced damage to the rodent gastric mucosa was reproduced by a single intragastric administration of indomethacin in a dose of 60 mg/kg in 1 ml of saline. In the stomach homogenate the activity of free radical oxidation was determined by conventional methods. For quantitative determination of cyclooxygenase-1, -2 in gastric homogenate the method of enzyme immunoassay was used. The concentration was determined spectrometrically by the color intensity of the samples. Results. With a prophylactic administration of tripeptide Leu-Ile-Lys, concentration of tiobarbiturate-reactive products was normalized, total antioxidant activity increased and the activity of antioxidant enzymes decreased compared to the control group. The concentration of cyclooxygenase-2 in gastric homogenate from the experimental animals was 2.3 times lower than that of the control rats. Conclusion. Use of Leu-Ile-Lys tripeptide in experimental indomethacin-induced gastric ulcer showed a significant decrease of oxidative damage and inflammation in the gastric mucosa.
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