Myoballs were cultured from neonatal rat skeletal muscle without the use of antimitotic drugs. Electron microscopic investigation showed that 7-day-old myoballs are multinucleated syncytia in a state of differentiation where filaments are abundant and already in hexagonal arrays. The resting potential of 142 myoballs kept at 20 degrees C was not correlated with the cell size. Its mean value was -64 mV. Cells with a high resting potential were capable of generating action potentials with a threshold of -51 mV, an overshoot of +31 mV, and a rate of rise of 100 V/s. The steady-state current-voltage relation showed inward rectification on hyperpolarization and outward rectification on depolarization. The dynamic sodium and potassium currents were investigated at 37 degrees C with the whole-cell-recording technique. The sodium current had its maximum at -20 mV. The potassium current showed delayed activation and a very slow and incomplete inactivation. The electrophysiological results from these cultured cells are very similar to those obtained from adult cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.