INTRODUCTION: This study has been carried out on reviewing the use of new synthetic component of zinc oxide nanoparticles (ZnO NPs) coated by Chitosan-linoleic acid (CS-LA) and to assess Minimum inhibitory concentration (MIC) of nanoparticles on clinical samples and biofi lm formation in vitro. MATERIALS AND METHODS: At fi rst the synthesized ZnO NPs coated by CS-LA were identifi ed with X-ray powder diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM) and Fourier Transform Infrared Spectroscopy analysis (FTIR).Through in vitro tests, the value of MIC and Minimum fungicide concentration (MFC) of nanoparticles and standard and clinical strains of candida were evaluated in comparison with fl uconazole as the control group using the CLSI-M27 method. Finally, biofi lm formation was studied using MTT assay. RESULT: The results showed that MIC50 of fl uconazole and nanoparticle in clinical strains was 64 μg/ml and 128 μg/ml, respectively. The MIC of fl uconazole and nanoparticle in C. albicans (ATCC10231) was 8 μg/ml and 32 μg/ml respectively. The MFC of nanoparticle and fl uconazole for clinical samples was recorded at similar level (128 μg/ml). MTT results indicated that the capacity of inhibition of biofi lm formation was 43.07 % and 36.68 % by ZnO NPs and fl uconazole, respectively. CONCLUSION: It is concluded that the new synthesized nanoparticle has appropriate effi cacy compared with fl uconazole in inhibitory activity on C. albicans growth and biofi lm formation. As a result, ZnO NPs can be introduced as an effective agent for diminishing adhesion capacity of C. albicans (Tab. 1, Fig. 4, Ref. 26).