In vitro studies were conducted to find out most efficient phosphate solubilizers. The 23 fungal and 9 bacterial phosphate solubilizing microorganisms were isolated from rhizospheric soil of different weeds occuring in sorghum and cotton crops by serial dilution method. Most efficient 'P' solubilizers were identified on the basis of halo zone formation on Pikovskaya's agar medium, reduction in pH, organic acid production, and P 2 O 5 solubilized in broth culture. The result indicated that among fungi Aspergillus niger-20 and Aspergillus niger-5 and among all bacterial isolates Dr. PDKV strain of PSB and PSB-3 produced maximum halo zone (5.33 to 4.66 mm) and they solubilized more tricalcium phosphate i.e. 26.24 to 18 P 2 O 5 µg/ml with reduction in pH (3.1 to 3.30) with increasing in titrable acidity i.e. 3.60 to 3.0. PSB-3, PSB-4, PSB-6 and PSB-8 produced indole acetic acid (IAA).
The investigation was undertaken to estimate fusaric acid from culture filtrate of Fusarium udum by using thin layer chromatography. Acidic pH (3.00), temperature 28 to 30°C and 20 days after incubation was found to be optimum for maximum fusaric acid production.
An experiment was conducted at Department of Horticulture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, during kharif 2019 to study the effect of biofertilizer, liquid organic manures viz., Panchagavya, Vermiwash, Jeevamrit and Cow urine on economic yield of okra in the open field condition. The experiment was laid in Randomized Block Design with fifteen treatments replicated twice by using the variety Parbhani Kranti. The various types of liquid organic solutions prepared from plant and animal origin are effective in promotion of growth and increasing yield in okra. Different concentration of liquid organic manures like Panchagavya (500 lit/ha), Vermiwash (100 lit/ha) and Cow urine (100 lit/ha) were given at 15, 45 and 75 DAS through foliar spray and Jeevamrit (500 lit/ha) and biofertilizer like Azospirillium (2.5 lit/ha) was applied through drenching at 15, 45 and 75 DAS along with Recommended dose of fertilizers (N:P:K::100:50:50]. The observations on yield parameters were recorded during the investigation. On the basis of observations recorded, the result of the present investigation revealed that, combined application of different organic liquid manures along with recommended dose of fertilizer has significant effect on economic yield of okra as compared to RDF alone. The yield parameter like weight of fruit (14.15 g), length of fruit (12.97 cm), diameter of fruit (14.81 mm), number of fruit per plant (20.73), fruit yield per plant (293.32 g) and fruit yield per ha (162.95 q/ha) were significantly superior in the treatment T14 [RDF + Jeevamrit + Vermiwash + Panchagavya] and it was found to be significantly superior over other treatments and gives best results as compared to Control (100:50:50 N:P:K kg/ha).
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