A convenient method for determining mixed linked 1,3:l,4-~-glucans in beer, wort, malt and barley is described. The fluorochrome Calcofluor, specific for ~-glucosidic linkages, is used to stain these materials. The fluorescence intensity of the final suspension is measured and the result compared with traditional ~-glucan measurements.The correlation between fluorescence intensity and (NH4)2SO4 precipitation was very good for beer and wort. The correlation between the fluorescence method and an enzymatic method used for malt and barley was somewhat poorer.

A fluorescence method is described for the qualification and quantification of bones and bone fragments in fish fillets. Bones from six different fish species were shown to exhibit a strong ultraviolet-blue fluorescence (390 nm), when excited by light at 340 nm. The method greatly simplifies searching for bones in fish fillets when they are not deeply embedded in tissue. It is concluded that the method offers promise in the establishment of a routine analysis for inspection of individual fillets in a normal filleting line for bones and parasitic nematodes.

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