A soil-free in vivo laboratory technique is described for the detection of systemic anti-fungal activity against Bremia lactucae, a representative of the Peronosporales. This system is not subject to some of the complications encountered with in vitro and glasshouse experiments. Two new compounds (prothiocarb and LS 73-1038 (Nina)) were found to have high systemic activity against B. lactucae using this technique and it was confirmed that zineb also has some systemic effect. Streptomycin sulphate and DPX-3217 showed some activity but were phytotoxic. Dowco 269 was phytotoxic and showed no activity against B. lactucae. None of these chemicals showed any significant vapour activity. (Crute & Johnson, 1976) was used throughout the study and cultures were maintained by mass spore transfers on seedlings of lettuce ( h t u c a satiwa L.) cvs Emerald or Mildura (R 3,4) using the method described by Dickinson & Crute (1974). Test plants.The cultivar for all tests was Unrivalled, a summer butterhead type considered to be universally susceptible to B. lactucae.Fungicides. Six chemicals were investigated in this study. Four of these were new compounds, reported to have systemic activity against members of the Peronosporales and released for trial purposes by chemical firms:
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