The air-dry tuflorescences (5 kg) of Rh. integrifolium C. Winkl., family Compositae collected at the end of flowering (radzhlk SSR, Darvazskii range, environs of the village of Sued) were extracted with 50 liters of methanol. After evaporation of the solvent, 360 g of a viscous resinous mass of extractive substances was obtained. According to TLC [SiO 2, chloroform-methanol (4 : 1)], the extract contained at least four phytoecdysones. Part of the total extract (230 g) was dissolved in 600 ml of methanol-water (2:1), and the hydrophobic compounds were extracted with benzene. The aqueous methanolic fraction was extracted with ethyl acetate. By chromatography on silica gel with elution by chloroform-methanol (9 : 1), the ethyl acetate extract yielded 3.2 g (0,10%) of a crystalline phytoecdysone, C27H140?, mp 235-236°C (ethyl acetate-meLhanol), [~]~+62.3 ° (c 1.54; CH3OH); A C2HsOH 244 nm (log ~ 4.057; vmK-Ba~ 3370-3520 (OH), 1660 [(:O~:ll inax C I cm -1. NMR spectrum at 100 MHz (CsHsN, internal standard HMDS, 5, ppm): 0.94
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