Previous attempts to determine ethylene oxide are surveyed and critically investigated. New methods of analysis have been suggested and certain modifications of existing methods applied to suit conditions of experiments. New methods for the detection and approximate determination of the fumigant in an atmosphere containing the gas have been established. Methods of recovery of ethylene oxide from grain and sultanas have been studied; part of the oxide has been shown to be readily recoverable and the remainder only with great difficulty.The relation between water content in the fumigated substance and the amount of fumigant retained has been studied, on the supposition that ethylene oxide reacts with the water in the material to form ethylene glycol. However, attempts to isolate and determine the glycol in the fumigated substance have failed. Various methods for the recovery of the firmly held portion of ethylene oxide have been devised and a procedure is described by which 92% of the total fumigant added can be recovered and determined.Contrary to the opinion of many authors, ethylene glycol is not formed in fumigated grain ; practically the whole of the fumigant is retained as ethylene oxide. It seems likely that a small proportion not exceeding 8% is combined with some constituents of the fumigated substance ; the proportion so combined depends on the period of contact and on the temperature to which the fumigated substance is subjected.
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