The effects of anti‐asialo GM‐1 antibody (AAGM) treatment on the engraftment of human T‐cell leukemia virus type I (HTLV‐I)‐infected human T cells in severe combined immunodeficiency (SCID) mice were studied. The frequency of tumor formation in an HTLV‐I‐transformed human T‐cell line, MT‐2 cells, at the site of inoculation was significantly higher in AAGM‐treated than untreated mice (P<0.05): 16/18 (89%) and 16/26 (62%), respectively. The promotive effect of AAGM treatment on tumor development was marked in the early stage (less than 3 weeks), suggesting that the immediate reaction of natural killers to the inoculated cells may be important for the prevention of tumor development. The surface phenotypes and clonality of the tumor cells were the same as the MT‐2 cells inoculated. Inoculation of peripheral blood mononuclear cells (PBMC) from one of the 4 adult T‐cell leukemia/lymphoma (ATL) patients resulted in the development of tumors in AAGM‐treated SCID mice. However, the surface phenotypes of the cells from these tumors were a mixture of B cells and T cells, suggesting that these tumors consisted of Epstein‐Barr virus‐transformed B cells and HTLV‐I‐transformed T cells. In addition, HTLV‐I was detected by polymerase chain reaction in various organs of the mice inoculated with PBMC from the ATL patient and the asymptomatic carrier examined. These results suggest that elimination of natural killer function by AAGM treatment is important, although such treatment is not always necessary for the engraftment of HTLV‐I‐infected cells in SCID mice.
The nature of the antibodies responsible for lupus erythematosus (LE) cells in systemic lupus erythematosus (SLE) remains obscure. Weexamined whether polyanion-restricted anti-histone antibodies were present in serum of patients with SLEusing Western blotting analysis. Dextran sulfate or alginate was used as a polyanion compoundin place ofDNA. Antibodies which recognized dextran sulfate-histone complexes were present in serum of patients with SLE (17/34, 50% ). These antibodies were detected in most SLE patients positive for LE cells (17/18, 94%) but not in those negative for LE cells or in patients with other collagen diseases. Similar results were obtained using alginate-histone complexes as antigens for Western blotting analysis. The antibodies to dextran sulfate-histone or alginate-histone complexes in serum of SLEpatients were completely absorbed by treatment of serum with DNA-histone complexes, while they were unaffected by treatment with DNAonly. The presence of antibodies to free histones and dextran sulfate-histone complexes did not seem to be related to the titer of anti-single stranded DNAantibody and anti-double stranded DNAantibody. We demonstrated the presence of polyanion-restricted antibodies in SLE, which may be responsible for the LE factor. (Internal Medicine 36: 781-786, 1997)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.