animals with various kinds of methods which differ in fixing and staining reactions from one another. I n the present paper we intend to account for the following points: (1) the distribution and arrangement of the nerves and ganglia of the pancreas ; (2) the peripheral nerve extension ; (3) the interrelation between the extrinsic nerve fibers and the intrinsic nerve elements. For this purpose we have treated the pancreas of the mouse with four kinds of nerve staining techniques and in addition undertaken degeneration experiment after section of the vagus. We propose to set forth o6r own observations and later to deal briefly with the related literature.
MATERIALS AND METHODSThe investigations undertaken were carried out on the pancreas of adult mice (Mus wagfieri uar. albula). Besides ob-331
SIXTY-ONE FIGURESThe pulmonary innervation in mammals has been a subject of discussion for many years. The conflicting results reported by many authors may be due, in part, to the capriciousness of the previous nerve staining techniques and, in part, to different techniques used by the various authors. From this point of view it will be advisable to study the same species of animals by various methods. In order to follow up the innervation of the lower respiratory organs of the mouse, in a recent paper (Honjin, '54) the writer has contributed a complete topographical survey of the tracheal and pulmonary nerve branches of both the vagus and the sympathetic, including their distribution with reference to the situation of the tracheal, hilus, and intrapulmonary ganglia. I n the present paper, we intend to account for the detailed histology of the minute structure of the intrapulmonary ganglia and nerves, as well as that of the nerve terminations. For this purpose we have selected and modified a variety of nerve staining techniques which differ fundamentally from one another, in Sxing and staining reactions.
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TWENTY-FIVE FIQURESI n a recent contribution (Honjin, '54), a complete topographical survey of the ganglia and the extrinsic nerve branches in the lower respiratory organs of the mouse has been made by means of the reconstruction method of serial sections, whereby the writer has described and figured the pulmonary nerve branches derived from the vagi, the sympathetic and the atrial plexus. I n a subsequent paper (Honjon, '56) a description of the minute innervation of the lung of the mouse has been given, working with a variety of staining techniques, in which the distribution and terminations of the efferent and afferent nerve fibers in the lung, as well as the correlation between the extrinsic nerve fibers and the local nerve cells are demonstrated. The questions arise as to what extent the vagus participates in the nerve supply of the lung and whether the bilateral innervation of the lung by the vagus occurs or not. In order to solve these questions, it seems necessary to undertake a nerve section to produce secondary degeneration which would give decisive results concerning the distribution of vagal nerve fibers. The degeneration experiment was tried by Larsell and Mason ('21)
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