A decentralized approach to diagnostics can decrease the time to treatment of infectious diseases in resource-limited settings. Yet most modern diagnostic tools require stable electricity and are not portable. Here, we describe a portable device for isothermal nucleic-acid quantification that can operate with power from electricity, sunlight or a flame, and that can store heat from intermittent energy sources, for operation when electrical power is not available or reliable. We deployed the device in two Ugandan health clinics, where it successfully operated through multiple power outages, with equivalent performance when powered via sunlight or electricity. A direct comparison between the portable device and commercial qPCR (quantitative polymerase chain reaction) machines for samples from 71 Ugandan patients (29 of which were tested in Uganda) for the presence of Kaposi’s sarcoma-associated herpesvirus DNA showed 94% agreement, with the four discordant samples having the lowest concentration of the herpesvirus DNA. The device’s flexibility in power supply provides a needed solution for on-field diagnostics.

Elastocaloric refrigerators are a promising alternative to the vapor compression cycle because they do not require refrigerants with high global warming potential. However, these coolers have yet to achieve temperatures low enough to — for example — be used as standard household refrigerators. We built one-stage, two-stage, and three-stage elastocaloric cooling systems to determine if staging of the elastocaloric effect could significantly expand temperature span. Our three-stage system achieved 1.5 times the maximum temperature span of our single-stage system, and produced the highest temperature span of any elastocaloric device to-date at 28.3 °C, where previously the record was 19.9 °C. Interestingly, we found that multistage systems can achieve equivalent temperature spans but at higher coefficients of performance compared to similarly-constructed single-stage systems.

Resource-limited settings present unique engineering challenges for medical diagnostics. Diagnosis is often needed for those unable to reach central healthcare systems, making portability and independence from traditional energy infrastructure essential device parameters. In 2014, our group presented a microfluidic device that performed a solar-powered variant of the polymerase chain reaction, which we called solar thermal PCR. In this work, we expand on our previous effort by presenting an integrated, portable, solar thermal PCR system targeted towards the diagnosis of Kaposi’s sarcoma. We call this system KS-Detect, and we now report the system’s performance as a diagnostic tool using pseudo-biopsy samples made from varying concentrations of human lymphoma cell lines positive for the KS herpesvirus (KSHV). KS-Detect achieved 83% sensitivity and 70% specificity at high (≥10%) KSHV+ cell concentrations when diagnosing pseudo-biopsy samples by smartphone image. Using histology, we confirm that our prepared pseudo-biopsies contain similar KSHV+ cell concentrations as human biopsies positive for KS. Through our testing of samples derived from human cell lines, we validate KS-Detect as a viable, portable KS diagnostic tool, and we identify critical engineering considerations for future solar-thermal PCR devices.

Carbon nanomaterials, including few-layered graphene (FLG), were synthesized on high-purity copper and nickel wires in a microchannel within an alumina microcombustor with a methane/oxygen edge flame.The deposition occurred in 20 s with identifiable FLG Raman peaks. The FLG layers were characterized by Raman spectroscopy and scanning electron microscope (SEM) imaging. The data shows 5-8 layers can be formed on the wires in a microchannel. The versatility of the microcombustor platform for rapid deposition of carbon nanomaterials is also shown through demonstrations of formation of near-perfect graphite thin films.

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