No abstract

Mastitis is an economically important disease and its subclinical state is difficult to diagnose, which makes mitigation more challenging. The objectives of this study were to screen clinically healthy ewes in order to: (i) identify cultivable microbial species in milk, (ii) evaluate somatic cell count (SCC) thresholds associated with intramammary infection, and (iii) estimate relationships between udder and teat morphometric traits, SCC, and ewe productivity. Milk was collected from two flocks in early (< 5 d) and peak (30-45 d) lactation to quantify SCC (n = 530) and numerate cultivable microbial species by culture-based isolation followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS; n = 243) identification. Within flock and lactation stage, 11 to 74% (mean = 36%) of samples were culture positive. More than 50 unique identifications were classified by MALDI-TOF MS analysis, and Bacillus licheniformis (18-27%), Micrococcus flavus (25%), B. amyloliquefaciens (7-18%), and Staphylococcus epidermidis (26%) were among the most common within flock and across lactation stage. Optimum SCC thresholds to identify culture-positive samples ranged from 175 × 10 3 cells/mL to 1675 × 10 3 cells/mL. Ewe productivity was assessed as total 120-d adjusted litter weight (LW120) and analyzed within flock with breed, parity, year, and the linear covariate of log10 SCC (LSCC) at early or peak lactation. Although dependent on lactation stage and year, each 1-unit increase in LSCC (e.g., an increase in SCC from 100 × 10 3 cells/mL to 1000 × 10 3 cells/mL) was predicted to decrease LW120 between 9.5 and 16.1 kg when significant. Udder and teat traits included udder circumference, teat length, teat placement, and degree of separation of the udder halves. Correlations between traits were generally low to moderate within and across lactation stage and most were not consistently predictive of ewe LSCC. Overall, the frequencies of bacteria-positive milk samples indicated that subclinical mastitis is common in these flocks and can impact ewe productivity. Therefore, future research is warranted to investigate pathways and timing of microbial invasion, genomic regions associated with susceptibility, and husbandry to mitigate the impact of subclinical mastitis in extensively managed ewes.

No abstract

Feed intake restriction impacts both humans and ruminants in late gestation, although it is unknown whether this adverse maternal environment influences the microbiome of the reproductive tract, and through it, the colonization of the fetal gut. A 2 × 2 factorial design including a 70% feed intake restriction (feed restricted ‘FR’ or control diets ‘CON’) and mineral supplementation (unsupplemented ‘S−’ or supplemented ‘S+’) was used to analyze these effects in multiparous cows (n = 27). Vaginal swabs were obtained 60, 30, and 10 days prior to the estimated calving date, along with neonatal rumen fluid and meconium. Placental tissues and efficiency measurements were collected. Microbial DNA was extracted for 16S sequencing of the V4 region. Feed restriction decreased the diversity of the placental microbiome, but not the vagina, while mineral supplementation had little impact on these microbial communities. Mineral supplementation did improve the richness and diversity of the fetal gut microbiomes in relation to reproductive microbes. These differences within the placental microbiome may influence individual health and performance. Adequate maternal nutrition and supplementation yielded the greatest placental efficiency, which may aid in the establishment of a healthy placental microbiome and fetal microbial colonization.

No abstract

Objectives were to evaluate breed, heterosis (HI), and maternal effects on wool production in Rambouillet (R x R), Targhee (T x T), and reciprocal-cross (R x T and T x R) 1-yr-old ewes. Greasy fleece weights (GFW) were obtained at shearing and mid-side, britch, and whole-fleece core wool samples were collected to quantify average (A-FD) and CV of fiber diameter (CV-FD). Laboratory scoured yield (LSY) was quantified on core samples and used to estimate clean fleece weight (CFW). Single-born ewes had greater GFW (3.48 kg), greater CFW (2.11 kg), and lower mid-side CV-FD (17.5%) than multiple-born ewes (3.18 kg, 1.95 kg, and 18.1%, respectively; P < 0.01). Rambouillet-sired ewes had greater LSY than T-sired ewes (60.6 vs. 60.0%; P < 0.01), but no breed effects were detected for GFW or CFW. A sire breed x dam breed interaction effect was detected for A-FD at all locations (P ≤ 0.05). Reciprocal-cross performance indicated unfavorable HI for A-FD within mid-side (+0.34 μm), britch (+0.97 μm), and core samples (+0.42 μm; P ≤ 0.05) compared to purebred average. Greater mid-side and britch A-FD in R x T (22.8 and 25.2 μm) than T x R ewes (21.2 and 23.5 μm; P < 0.01) implied a more favorable additive maternal effect for crossbred ewes gestated and reared by R compared to T dams. Future analyses will consider lifetime lamb and wool production of these breed types to evaluate the utility of finewool crossbred ewes in extensive production systems.

No abstract

Subclinical mastitis is a common intramammary disease in sheep production systems. Expenses associated with compromised animal performance, therapeutic interventions, and decreased ewe longevity make efforts to minimize its prevalence worthwhile. The objectives of this study were to (i) quantify the prevalence of subclinical mastitis throughout lactation, (ii) evaluate the impact of bedding treatments on subclinical mastitis during early lactation, (iii) evaluate the efficacy of prophylaxis and feed restriction during weaning on subclinical mastitis cure rates, (iv) and identify levels and types of antimicrobial resistance in milk-derived bacteria. Ewe milk samples were collected at d 1, 2, and 28 post-partum, weaning, and 3-d post-weaning for bacterial identification via culture-based methods. Staphylococcus spp. and Streptococcus spp. isolates were subjected to in vitro antimicrobial susceptibility testing. The overall prevalence of subclinical mastitis defined by culture growth ranged between 22 and 66% and differences were observed between post-weaning and d 1 and 28 milk samples. Commonly isolated bacteria include coagulase-negative staphylococci (CoNS; 59%), Bacillus spp. (35%), Mannheimia haemolytica (10%), Staphylococcus aureus (8%), Streptococcus spp. (5%), and Corynebacterium spp. (5%). Early milk samples (d 1 and 2) were compared between jug bedding treatment: jugs were recently vacated, cleaned, and dusted with barn lime before adding fresh straw (CLEAN) or jugs were previously vacated and fresh straw was added atop soiled bedding (SOILED). Jug bedding treatment did not affect the prevalence of subclinical mastitis, though CoNS had greater sulfadimethoxine resistance in SOILED isolates than CLEAN isolates (P = 0.03). Three different weaning treatments were used: ewes were injected with penicillin at weaning (PENN), ewes had restricted feed access 48 h prior to and 72 h post-weaning (FAST), or a combination of these treatments (COMBO). Weaning treatment did not affect the prevalence of subclinical mastitis or cure rate from weaning to 3-d post-weaning, though all PENN and no FAST milk S. aureus isolates were resistant against tetracycline (P = 0.08). Subclinical mastitis prevalence tended to decrease from weaning to post-weaning (P = 0.08). These data show subclinical mastitis is common throughout lactation and the levels of antimicrobial resistance of bacteria isolated from ewe milk are generally low against commonly used antimicrobials.