The amount of experience with ultrasonography may influence measurement outcomes while images are acquired or analyzed. The purpose of this study was to identify the interrater reliability of ultrasound image acquisition and image analysis between experienced and novice sonographers and image analysts, respectively. Following a brief hands-on training session (2 h), the experienced and novice sonographers and analysts independently performed image acquisition and analyses on the biceps brachii, vastus lateralis, and medial gastrocnemius in a sample of healthy participants (n = 17). Test–retest reliability statistics were computed for muscle thickness (transverse and sagittal planes), muscle cross-sectional area, echo intensity and subcutaneous adipose tissue thickness. The results show that image analysis experience generally has a greater impact on measurement outcomes than image acquisition experience. Interrater reliability for measurements of muscle size during image acquisition was generally good–excellent (ICC2,1: 0.82–0.98), but poor–moderate for echo intensity (ICC2,1: 0.43–0.77). For image analyses, interrater reliability for measurements of muscle size for the vastus lateralis and biceps brachii was poor–moderate (ICC2,1: 0.48–0.70), but excellent for echo intensity (ICC2,1: 0.90–0.98). Our findings have important implications for laboratories and clinics where members possess varying levels of ultrasound experience.

Background: Aging results in adaptations which may affect the control of motor units. Objective: We sought to determine if younger and older men recruit motor units at similar force levels. Design: Cross-sectional, between-subjects design. Setting: Controlled laboratory setting. Participants: Twelve younger (age = 25 ± 3 years) and twelve older (age = 75 ± 8 years) men. Measurements: Participants performed isometric contractions of the dominant knee extensors at a force level corresponding to 50% maximal voluntary contraction (MVC). Bipolar surface electromyographic (EMG) signals were detected from the vastus lateralis. A surface EMG signal decomposition algorithm was used to quantify the recruitment threshold of each motor unit, which was defined as the force level corresponding to the first firing. Recruitment thresholds were expressed in both relative (% MVC) and absolute (N) terms. To further understand age-related differences in motor unit control, we examined the mean firing rate versus recruitment threshold relationship at steady force. Results: MVC force was greater in younger men (p = 0.010, d = 1.15). Older men had lower median recruitment thresholds in both absolute (p = 0.005, d = 1.29) and relative (p = 0.001, d = 1.53) terms. The absolute recruitment threshold range was larger for younger men (p = 0.020; d = 1.02), though a smaller difference was noted in relative terms (p = 0.235, d = 0.50). These findings were complimented by a generally flatter slope (p = 0.070; d = 0.78) and lower y-intercept (p = 0.009; d = 1.17) of the mean firing rate versus recruitment threshold relationship in older men. Conclusion: Older men tend to recruit more motor units at lower force levels. We speculate that recruitment threshold compression may be a neural adaptation serving to compensate for lower motor unit firing rates and/or denervation and subsequent re-innervation in aged muscle.

Echo intensity may associate with indicators of skeletal muscle quality, but investigators often use different methodological approaches that may alter echo intensity when acquiring B-mode ultrasound images. We examined the influence of image depth and gain settings on the interpretation of echo intensity. Thirty-six college-aged males and females participated. Ultrasound images of the vastus lateralis were captured in the sagittal plane. Images were captured at depths of 3.0, 3.5, 4.0, 4.5, 5.0, 6.0, and 7.0cm and gain settings of 50 and 60 dB in random order. For both gain settings, echo intensity values were similar between 4.0 and 6.0cm. At a gain of 50dB, 7.0cm demonstrated greater values than all other depths. At a gain of 60dB, 7.0cm displayed greater values compared to all other depths, but 3.0cm displayed lower echo intensity values than other depths. Echo intensity was substantially higher when using a gain of 60dB compared to 50dB (mean difference ≥ 21.7 arbitrary units, d ≥ 3.47). When planning investigations in new research areas, researchers should carefully consider their study-specific image depth. Echo intensity values are stable between depths of 4.0 and 6.0cm, suggesting that changing image depth may not be problematic. Image gain must be kept constant. Novelty Bullets • Optimal approaches for B-mode ultrasound image acquisition of skeletal muscles remain unclear. • Echo intensity is similar between depths of 4.0 and 6.0cm, regardless of image gain. • Investigators should seek to utilize a constant depth setting, but small deviations may be acceptable.

Low back pain (LBP) is a leading cause of disability. The use of deadlift-based assessments in assessing LBP is becoming common in clinical settings, but these concepts have not been well studied. We sought to compare force and muscle excitation during isometric deadlifts in participants suffering from LBP versus asymptomatic controls. We also compared these outcomes for conventional versus hexagonal barbells. Sixteen adults with mild-to-moderate, acute, non-specific LBP and 19 controls performed maximal, isometric deadlifts while standing on a force plate using conventional and hexagonal barbells. Surface electromyographic signals were recorded from the upper trapezius, external oblique, erector spinae, vastus lateralis, and biceps femoris. Normalized peak force and peak rate of force development were similar for those with acute, non-specific LBP and controls. Surface electromyographic excitation was not different between groups, but was higher with the hexagonal barbell for the vastus lateralis and upper trapezius. Both groups felt equally safe and confident. In summary, the presence of acute, non-specific LBP did not impair peak and rapid force or muscle excitation. Hexagonal barbells may optimize knee extensor and trapezius activation. Deadlift-based force assessments appear safe and may be useful in the assessment of functional strength in patients with acute, non-specific LBP.

Advances in surface electromyographic (EMG) signal decomposition allow researchers to analyze data for 20–50 motor units per contraction. To simplify interpretation, some investigators rely on group mean analysis of the mean firing rate versus recruitment threshold relationship, but it is unclear if this association is linear. Objective: To determine whether this relationship is strongest when analyzed via linear, quadratic, or cubic regression. Approach: Twenty-one men (mean  ±  SD age  =  24  ±  4 years) and 16 women (21  ±  2 years) performed isometric contractions of the knee extensors at 50% of maximal force while bipolar surface EMG signals were recorded from the vastus lateralis. A decomposition algorithm was used to calculate the mean firing rate and recruitment threshold of each motor unit at accuracy levels ranging from 90.0%–93.0%. Polynominal regression was used to determine if each relationship was best fit with a linear, quadratic, or cubic model. We examined individual contractions and grouped data. Main results: Overall, 80% of the relationships were best fit with a linear model. Quadratic and cubic relationships were more appropriate for 16% and 2% of the contractions, respectively. Selecting varying accuracy levels within a range of 90.0%–93.0% had little influence on whether a given dataset was best fit with a linear, quadratic, or cubic model. Grouping of data provided different relationships than otherwise found on a contraction-by-contraction basis. Significance: The mean firing rate versus recruitment threshold relationship is typically best fit with a linear model. These relationships should be examined on an individual contraction basis.