Protoplasts have been prepared from sporophytes of Laminaria digitala and Laminaria saccharina. Different culture media have been tested in order to achieve cell wall regeneration. The most appropriate culture medium for Laminaria digitata protoplasts was found to be an ionic medium with glucose as extra osmoticum. For Laminaria saccharina protoplasts, seawater supplied with glucose was more appropriate. The composition of alginates produced from regenerating protoplasts have determined by NMR and by an enzymatic method. Alginate composition is found to be markedly influenced by the medium composition.
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