This study demonstrated a positive impact of including FRM skills and confidence building in a nutrition education program, the effects of which could be seen for 6 months after participation in the program. Equipping low-income families with FRM skills allowed them to access healthier foods even during times of hardship.

Background Measurable residual disease (MRD) is a strong independent poor prognostic factor for acute leukemia. Multiparameter flow cytometry (FCM) is a commonly used MRD detection method. However, FCM MRD detection is not well standardized, and the interpretation is subjective. There are normal/reactive minor cell populations in bone marrow (BM) and peripheral blood (PB), which could be confused with MRD. Methods The FCM data of 231 BM and 44 PB pediatric samples performed in a recent 15‐month period were retrospectively reviewed. These samples were from 56 B‐lymphoblastic leukemia (B‐ALL) patients, 11 T‐lymphoblastic leukemia (T‐ALL) patients, 28 acute myeloid leukemia (AML)/myelodysplastic syndrome (MDS) patients, 44 cytopenia/leukocytosis patients, and five patients with mycosis fungoides. Results There were over 10 normal or reactive minor cell populations identified with certain phenotypes mimicking MRD of acute leukemia. These mimickers included CD19+ NK cells, CD22+ basophils, CD22+ dendritic cells (DCs), and plasma cells for B‐ALL MRD; CD4/8 double‐negative T cells, CD4/8 double‐positive T cells, cytoplasmic CD3+ NK cells, CD2− T cells, CD7− T cells, CD5− gamma delta T cells, CD56+ NKT cells for T‐ALL MRD; CD33+ NK cells, CD117+ NK cells, basophils, plasmacytoid DCs, non‐classical monocytes, CD56+ and/or CD61+ monocytes for AML MRD. Conclusions These data confirm the presence of a variety of normal/reactive minor cell populations that could mimic MRD of acute leukemia by FCM. Recognizing these MRD mimickers is important for correct FCM MRD interpretation.

Clinical use of nitric oxide (NO) is usually in conjunction with high oxygen concentrations, the effects of which may include lung neutrophil accumulation, apoptosis and upregulation of antioxidant enzyme activity. To define the effects of NO on neutrophils from young piglets and its relationship to lung neutrophil dynamics during hyperoxia we exposed thirty piglets to room air (RA), RA+NO (50 ppm NO), O₂ (FiO₂≥0.96) or O₂+NO for 5 days. Ten additional animals breathed RA+NO or O₂+NO, then recovered in RA for 3 days before sacrifice. Neutrophil CD18 and intracellular oxidant production were measured by flow cytometry. Lung apoptosis were assessed by TUNEL assay. Lung myeloperoxidase, SOD and catalase were measured biochemically. When compared to RA group, there was significant reduction in neutrophil CD18 and intracellular oxidant production in the RA+NO group, but lung MPO was unchanged. The O₂ and O₂+NO groups did not differ in CD18 expression or in intracellular oxidant production, but had significant increase in lung myeloperoxidase compared to the RA group. Apoptosis increased significantly only in the O₂+NO group. The O₂ group showed significantly increased lung SOD and catalase activity compared to the RA group, whereas the RA+NO and O₂+NO groups did not. We conclude that inhaled NO at 50 ppm decreases neutrophil CD18 expression as well as intracellular oxidant production. However, this effect does not impact lung neutrophil accumulation during concurrent hyperoxia. The combination of NO and O₂ exposure produces an increase in lung apoptosis. Finally, NO may prevent upregulation of SOD and catalase activity during hyperoxia, potentially increasing injury.

To explore the effects of the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) on participants' breastfeeding (BF) practices, studies that use large national samples are warranted. The US Department of Agriculture's Food and Nutrition Service funded several studies that leveraged national samples of WIC agencies, sites, and participants to evaluate how WIC affects BF outcomes among its participants. This report integrates findings across multiple Food and Nutrition Service-funded studies to describe important program benefits and how the program and its benefits supported positive attitudes and beliefs toward BF, as well as higher rates of BF initiation. The report concludes with policy implications and suggestions for future research.

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