A. Introduction. The work reported in this paper had for its object a, comparative study of three of the most valuable methods of determining the hydrogen-ion concentration of the blood, and arose from the development of the glass electrode technique (Brown(l), Kerridge(2)), which, from the point of view both of its accuracy and of its convenience in use, seemed an appropriate standard with which the Dale-Evans colorimetric method might be compared, particularly since a certain amount of controversy has arisen as to the accuracy of this last method (for literature see L e pp e r and Martin (3)). For the sake of completeness, and in order to check any such consistent errors as might arise, (i) from loss of C02, or (ii) from the depolarisation of the hydrogen electrode through reduction of C02 by the platinum black, the use of this electrode also was included in the comparison. The results given thus serve to determine both systematic differences (if any such exist) between the three methods, and also their individual instrumental and observational errors; they are an amplification of results already published by two, of us(4).B. Technique. (1) Hydrogen electrode. The chief difficulty in making accurate measurements on blood with the hydrogen electrode is the necessity of complete elimination of oxygen, and in this series of experiments the blood was always carboxylated by preliminary saturation with a hydrogen-carbon monoxide mixture. In this way the blood was very easily and completely saturated with hydrogen in the electrode vessel, the measurements with the glass electrode and with the colorimetric method were not upset by drifts due to a slow absorption of oxygen, and the readings with the hydrogen electrode were not rendered inaccurate by a somewhat doubtful correction for the change in reaction with oxygenation.
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