The impact of nematode consumption on aboveground net primary production (ANPP) was addressed by treating two areas of mixed-grass prairie with the nematicide carbofuran. Monthly assessments of shoot biomass and nematode populations were made from each treated and control site for two growing seasons. Seasonal mean density of root-feeding nematodes was reduced approximately 82% by carbofuran. ANPP was significantly greater in the treated plots for both seasons in Site 1 but only for the second year in Site 2. Increases in ANPP averaged 51% in Site 1 and 26% in Site 2. It was estimated that root-feeding nematodes reduced ANPP by 16 times more than they consumed.
Columbia root-knot nematode, Meloidogyne chitwoodi, parasitizes potato plants and causes visible small brown dots in the tuber flesh that dramatically reduce the market value of the crop. In the Pacific Northwest (PNW), two races of M. chitwoodi, Race 1 and Race 2 and a pathotype Race 1Roza exist. The races and pathotype of M. chitwoodi are primarily differentiated based on host tests. Currently, M. chitwoodi can be differentiated from M. hapla or other Meloidogyne Sp. based on morphology and by molecular markers however, the different pathotypes of M. chitwoodi cannot be differentiated using the aforementioned techniques. Based on the in silico genome comparison of M. chitwoodi Race 1, Race 2 and Race 1Roza, we developed diagnostic molecular markers that could successfully differentiate between M. chitwoodi races and pathotype. Further, we also developed three race-specific markers that can specifically amplify Race 1, Race 2, or Race 1Roza. Overall, we have potential markers that can successfully differentiate all the two races and one pathotype of M. chitwoodi through simplex PCR, which has potential application in plant disease diagnostics.
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